A yeast geneticist irradiates haploid cells of a strain thatis an adenine-requiring auxotrophic mutant, caused bymutation of the gene ade1. Millions of the irradiatedcells are plated on minimal medium, and a small number of cells divide and produce prototrophic colonies.These colonies are crossed individually with a wildtype strain. Two types of results are obtained:(1) prototroph × wild type : progeny all prototrophic(2) prototroph × wild type : progeny 75% prototrophic,25% adenine-requiring auxotrophsa. Explain the difference between these two types ofresults.b. Write the genotypes of the prototrophs in each case.c. What progeny phenotypes and ratios do you predictfrom crossing a prototroph of type 2 by the original ade1auxotroph?

Human Anatomy & Physiology (11th Edition)
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A yeast geneticist irradiates haploid cells of a strain that
is an adenine-requiring auxotrophic mutant, caused by
mutation of the gene ade1. Millions of the irradiated
cells are plated on minimal medium, and a small number of cells divide and produce prototrophic colonies.
These colonies are crossed individually with a wildtype strain. Two types of results are obtained:
(1) prototroph × wild type : progeny all prototrophic
(2) prototroph × wild type : progeny 75% prototrophic,
25% adenine-requiring auxotrophs
a. Explain the difference between these two types of
results.
b. Write the genotypes of the prototrophs in each case.
c. What progeny phenotypes and ratios do you predict
from crossing a prototroph of type 2 by the original ade1
auxotroph?

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