A yeast geneticist irradiates haploid cells of a strain thatis an adenine-requiring auxotrophic mutant, caused bymutation of the gene ade1. Millions of the irradiatedcells are plated on minimal medium, and a small number of cells divide and produce prototrophic colonies.These colonies are crossed individually with a wildtype strain. Two types of results are obtained:(1) prototroph × wild type : progeny all prototrophic(2) prototroph × wild type : progeny 75% prototrophic,25% adenine-requiring auxotrophsa. Explain the difference between these two types ofresults.b. Write the genotypes of the prototrophs in each case.c. What progeny phenotypes and ratios do you predictfrom crossing a prototroph of type 2 by the original ade1auxotroph?
A yeast geneticist irradiates haploid cells of a strain that
is an adenine-requiring auxotrophic mutant, caused by
mutation of the gene ade1. Millions of the irradiated
cells are plated on minimal medium, and a small number of cells divide and produce prototrophic colonies.
These colonies are crossed individually with a wildtype strain. Two types of results are obtained:
(1) prototroph × wild type : progeny all prototrophic
(2) prototroph × wild type : progeny 75% prototrophic,
25% adenine-requiring auxotrophs
a. Explain the difference between these two types of
results.
b. Write the genotypes of the prototrophs in each case.
c. What progeny
from crossing a prototroph of type 2 by the original ade1
auxotroph?
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