a) What is the main purpose of using “Controlled Heat Treatment Procedure”? b) What are the main criteria to establish proper process conditions? Please explain in detail
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4. a) What is the main purpose of using “Controlled Heat Treatment Procedure”?
b) What are the main criteria to establish proper process conditions? Please explain in detail
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- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropper5) Aseptic technique refers to A) the microbial inoculum placed into a test tube or onto a Petri plate. B) a series of practices to avoid contamination. C) the autoclave and other sterilizing procedures. D) cleanliness in the laboratory.Given the following common laboratory materials, which of the three methods of heat sterilization do you think is the most appropriate and practical to use in sterilizing them ? Why do you think so ? a) empty flask b)L-rod (glass rod c)10 ml glass pipet d) wire needles e)antibiotic solution
- Clinical thermometer can be sterilized by boiling. a) True b) False1.) During a laboratory activity, Chemical reactions from mixture of chemicals should be observed under which laboratory equipment? a.) autoclave b.) fume hood c.) Labaratory Safety cabinet d.) Centrifuge 2.) Which type of equipment contains a HEPA filter that only removes suspended materials from exhausted air? a.) Fume hood b.) Biosafety Cabinet I c.) Biosafety Cabinet II d.) Biosafety Cabinet IIIOther than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.
- 11. ● Possible methods for the quantitative determination of sodium bicarbonate for injection are: A) Acidimetry B) Alkalimetry C) Complexometry D) Iodometry E) Refractometry ● Calcium chloride is available in dosage forms: A) Powder for preparation of injections B) Solution for injection 10% in ampoules C) Tablets of 0.3 g D) Solution for injection 25% in ampoules E) Dry dosed powderA) Draw and label the set-up of Manual paraffin wax infiltration. B) Answer the following questions:1. Differentiate the 3 types of tissue Impregnation in terms of: 2. Impregnation Techniques Advantages Disadvantages A. Paraffin Infiltration B.Celloidin Infiltration C.Gelatin Impregnation C) What is the required volume of embedding medium for routine tissue processing? D) What is Plastic embedding? What is Double embedding?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?
- A) Sterile compounding facilities must use the garbing order specified in the USP standards. B) A gown may be reused for an entire shift if not visibly soiled. C)Staff are evaluated on garbing technique using surface sampling. D) Gloves should be donned inside of the laminar airflow workbench (LAFW).6) The autoclave desired Temperature to kill bacterial spores must be left for at least ____ in order to effectively kill the bacterial spores: a) 5 mins b) 10 mins c) 15 mins d) ½ hr e) 1 hr.7) The autoclave pressure must reach at least ________ in order to effectively kill the bacterial spores: a)15 PSI b) 15 Joules c) 15 KSI d) 15 Lbs e) 15 Atmospheres8) The antibiotic that inhibits the cell wall formation through the -Lactam ring is/are:a) Vancomycin b) Tetracycline c) Penicillin d) Cephalosporin e) c & d9) The sum of all chemical reactions in your body defines: a) anabolism b) metabolism c) catabolism d) Cellular Respiration e) Biosynthesis10) If in a bacterial growth medium waste product accumulates than the bacterial growth stage will be called: a) Death b) Convalescence c) Lag phase d) Log e) StationaryList 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?