a) Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) can be used as examples to explain difference between adsorption and partition. For each of these chromatographic techniques, identify: + the stationary and mobile phase + how the mobile phase moves b) How is the Rf value for a spot on a TLC plate calculated? What can the Rf value be used for? In order to achieve the best separation of several components of different polarity in a mixture spotted on a TLC plate, what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc.)

a) Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) can be used as examples to explain difference between adsorption and partition. For each of these chromatographic techniques, identify: + the stationary and mobile phase + how the mobile phase moves b) How is the Rf value for a spot on a TLC plate calculated? What can the Rf value be used for? In order to achieve the best separation of several components of different polarity in a mixture spotted on a TLC plate, what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc.)

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter5: Biomedical Electronics: Digital

Section: Chapter Questions

Problem 5Q

See similar textbooks

Similar questions

a) What are the important parameters for the eluent selection in Gel Permeation Chromotography (GPC)? Explain your answer in detail. What is an universal calibration curve and what is the advantage of using it in GPC analysis?
Describe two different applications of superparamagnetic particles in bio-medicines and what are the advantages of using superparamagnetic particles.
3. Which of the following statements is correct about column chromatography? a) Resolution improves as column length increases b) Mobile phase is a porous solid material kept in the column with appropriate chemical characteristics c) Stationary phase is a buffered solution that percolates through the mobile phase d) Large proteins exit the column faster than tiny proteins
Explain this approach: matrix-assisted laser desorption ionization (MALDI) ?
In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.
Regarding the use of animal cells in culture, it is correct to state that: * (a) Low cost culture media composed of mineral salts and amino acids are used (b) Cells must be kept in semi-solid media, allowing them to come into contact with the atmosphere rich in carbon dioxide. (c) Greenhouses with controlled temperature and atmosphere composed predominantly of carbon monoxide are used (d) On an industrial scale, culture media supplemented with serum of animal origin are used, which makes them media of indefinite composition (e) Culture media must not be sterilized by autoclaving, preferably filtration and/or ionizing radiation methods are used
Discuss the principles and applications of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry in molecular diagnostics.
Name two radioisotopes used within the medical imaging processes, one administered by inhalation and one used by intravenous injection. For both of these radioisotopes, what are their half-life and what is the relevance of that to why it is used? What are both of these specific use in diagnostic imaging?What are they treating?
You are asked to design an assay to measure the concentration of WonderDrug in various different pharmaceutical preparations using either its UV-visible absorption or its fluorescence emission. Explain when a UV-visible absorption based assay would be the more appropriate and when a fluorescence based assay would be more appropriate.
Elaborate the essential differences between Molecular beam epitaxy and AACVD
Answer this ff question: 1. Explain the importance of system suitability on HPLC method of analysis. 2.In UV and visible spectrophotometry, the specimen is generally dissolved in a solvent, and determinations are made at room temperature using a path length of 1 cm. Give the most common solvents suitable for UV or visible spectrophotometry.
Which of the following technique is FALSE in microscopy? Osmium tetroxide can be used as a fixative as well as a negative stain in transmission electron microscopy. Two proteins; each tagged with their individual primary antibody followed by one protein with rhodamine conjugated secondary antibody and the other with fluorescein-conjugated secondary antibody can be visualized simultaneously using a fluorescence microscope. The emission spectrum of Rhodamine is 580 nm The emission spectrum of Fluorescein is 521 nm O An objective lens with 100X magnification and projection lens with 10X magnification is going to produce a 1000X total magnification. Phase contrast and differential interference contrast (DIC) do not require staining.