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Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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![A purified protein is in a HEPES (N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)) buffer at pH 7 with
600 mM NaCl. A 1 mL sample of the protein solution is placed in a tube made of dialysis membrane and dialyzed against
2.0 L of the same HEPES buffer with 0 mM NaCl. Small molecules and ions, such as Na, Cl, and HEPES, can diffuse
across the dialysis membrane, but the protein cannot.
Once the dialysis has come to equilibrium, what is the concentration of NaCl in the protein sample? Assume no volume
changes occur in the sample during the dialysis.
[NACI] =
mM
If the original 1 mL sample were dialyzed twice, successively, against 100 mL of the same HEPES buffer with
O mM NaCl, what would be the final NaCl concentration in the sample?
[NaCl] =
mM](https://content.bartleby.com/qna-images/question/18593a3a-b463-4aeb-a821-a472ffee6b36/38f616a2-caed-42cf-a318-3afa53b3c827/k2a8fow_thumbnail.png)
Transcribed Image Text:A purified protein is in a HEPES (N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)) buffer at pH 7 with
600 mM NaCl. A 1 mL sample of the protein solution is placed in a tube made of dialysis membrane and dialyzed against
2.0 L of the same HEPES buffer with 0 mM NaCl. Small molecules and ions, such as Na, Cl, and HEPES, can diffuse
across the dialysis membrane, but the protein cannot.
Once the dialysis has come to equilibrium, what is the concentration of NaCl in the protein sample? Assume no volume
changes occur in the sample during the dialysis.
[NACI] =
mM
If the original 1 mL sample were dialyzed twice, successively, against 100 mL of the same HEPES buffer with
O mM NaCl, what would be the final NaCl concentration in the sample?
[NaCl] =
mM
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