When analyzing urine from a patient with kidney disease, which organic molecules might be detected? Explain. Which biochemical test will be the most appropriate for detection of those molecules?

Consider a crude extract with a mixture of the 5 proteins listed below. Protein A 4.5 pl 160 molecular weight, kDa Protein B 12.5 pl and 65 molecular weight Protein C 5.0 pl, 15 molecular weight Protein D 6.8 pl, 150 molecular weight Protein E 9.5 pl, 45 molecular weight You load this protein mixture onto an anion exchange column at pH 11. Next, you apply a "washing" step by passing through buffer at pH 11. Finally, for your elution step, you apply a pH gradient starting from pH 11 to pH 2.0 (A gradient buffer system allows you to gradually and continuously change the pH of your mobile phase starting from pH 11 up to pH 2). You load this same protein mixture onto a Size Exclusion column. Please indicate the order in which these proteins will elute for both. Group of answer choices Yes or No, please explain your answer. If your protein of interest is protein A, would using anion exchange column be completely successful at separating it from all the other proteins? Group of answer…

Given a stock protein solution with a concentration of 6 mg/ml, determine the protein concentration of a solution made by mixing 5 μl of the stock with 5 μl of a buffer.

Cottage cheese and many other cheeses are produced by adding the enzyme rennin to milk, producing curds and whey. a) Design an experiment to determine the pH at which rennin is most effective. How will you measure the results? b) What are tests that can be used to detect protein, sugars, and fats? How can these tests to determine the chemical makeup of curds and whey?

A patient is suspected of having low stomach acid, a condition known as hypochloridia. To determine whether the patient has this condition, her doctors take a 18.00 mL sample of her gastric juices and titrate the sample with 3.94 x 10-4 M KOH. The gastric juice sample required 6.60 mL of the KOH titrant to neutralize it. Calculate the pH of the gastric juice sample. Assume the sample contained no ingested food or drink which might otherwise interfere with the titration. pH = Enter numeric value For the patient to be suffering from hypochloridia, the pH of the gastric juices from the stomach must be greater than pH 4. Does the patient have hypochloridia? no O unable to determine O yes

In protein electrophoresis, what reagent present  in the sample buffer is used to eliminate differences in the charge densities of proteins?   Tris-HCL ph 8.8   2-mecraptothanol   SDS(sodium dodecyl sulfate)   Tris-HCL PH 6.8

Are Xanthoproteic and MillonNasse tests satisfactory for use in the urinary examination for protein? Why? Which test can be used to show up to what stage the hydrolysis of a protein proceeds? Why?

Concentrations in biochemical systems are often very dilute. Consequently, scientific notation and logarithms are often used to express concentrations. In scientific notation, numbers are expressed as coefficient x 10" To convert a number to scientific notation, proceed as follows: 1. Move the decimal place so that there is one digit in front of the decimal. 2. Account for the moved decimal in the value of x. If the decimal moved to the right, x is negative; if it moved to the left, x is positive. A logarithm is basically an exponent. Unless otherwise indicated, a logarithm is the a of 10". The numbers after the decimal point are significant; the number before the decimal just identify the location of the decimal point for the number. Notice that it is easy to estimate a logarithm from scientific notation; it's the exponent! Logarithms are commonly used to express the concentration of H. The pH is defined as pH= log (In), where the base number is 2.303. The same general rules as logs…

A purified protein fraction has a total sample volume of 450 µL. The sample has a corrected A280 of 0.244, and the blank corrected A280 was 0.077. (Both values were measured with a path length of 1.00 cm.) If 5.50 µL of the sample was used in a reaction, calculate the mass of protein in the reaction (in µg).

A purified protein fraction has a total sample volume of 360 µL.  The sample has a corrected A280 of 0.484, and the blank corrected A280 was 0.052.  (Both values were measured with a path length of 1.00 cm.)  If 5.00 µL of the sample was used in a reaction, calculate the mass of protein in the reaction (in µg).