A protein gives, under conditions of buffer composition, pH, and temperature that are close to physiological conditions, a molecular weight by size exclusion measurements of 140,000 g/mol. When the same protein is studied by SDS gel electrophoresis in the absence or presence of the reducing agent 3-mercaptoethanol (BME), the patterns seen, respectively, in lanes A and B are observed. Lane C contains standards of molecular weight indicated. cm migrated 0 2 10 A - BME B + BME с STD Serum albumin, 67,000 Ovalbumin, 43,000 Carbonic anhydrase, 30,000 Trypsin Inhibitor, 20,000 ▾ Part A Describe the native protein, in terms of the kinds of subunits present, the stoichiometry of subunits, and the kinds of bonding (covalent, noncovalent) existing between subunits. Match the items in the left column to the appropriate blanks in the sentences on the right. absence ionic four Mw 70,000 and Mw≈ 40,000 covalently hydrogen two identical Mw≈ 70,000 Mw 30,000 and Mw≈ 40,000 noncovalently presence Mw 70,000 and Mw 30,000 disulfide There are by the pattern of The native molecule contains the pattern of The in the in the Reset Help linked subunits that are suggested of BME. units that are suggested by of BME. units are paired by at least one bond.
A protein gives, under conditions of buffer composition, pH, and temperature that are close to physiological conditions, a molecular weight by size exclusion measurements of 140,000 g/mol. When the same protein is studied by SDS gel electrophoresis in the absence or presence of the reducing agent 3-mercaptoethanol (BME), the patterns seen, respectively, in lanes A and B are observed. Lane C contains standards of molecular weight indicated. cm migrated 0 2 10 A - BME B + BME с STD Serum albumin, 67,000 Ovalbumin, 43,000 Carbonic anhydrase, 30,000 Trypsin Inhibitor, 20,000 ▾ Part A Describe the native protein, in terms of the kinds of subunits present, the stoichiometry of subunits, and the kinds of bonding (covalent, noncovalent) existing between subunits. Match the items in the left column to the appropriate blanks in the sentences on the right. absence ionic four Mw 70,000 and Mw≈ 40,000 covalently hydrogen two identical Mw≈ 70,000 Mw 30,000 and Mw≈ 40,000 noncovalently presence Mw 70,000 and Mw 30,000 disulfide There are by the pattern of The native molecule contains the pattern of The in the in the Reset Help linked subunits that are suggested of BME. units that are suggested by of BME. units are paired by at least one bond.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
6-1.1
Transcribed Image Text:A protein gives, under conditions of buffer composition, pH, and
temperature that are close to physiological conditions, a molecular
weight by size exclusion measurements of 140,000 g/mol. When
the same protein is studied by SDS gel electrophoresis in the
absence or presence of the reducing agent 3-mercaptoethanol
(BME), the patterns seen, respectively, in lanes A and B are
observed. Lane C contains standards of molecular weight
indicated.
cm migrated
0
2
8
10
A
- BME
B
+ BME
с
STD
Serum albumin,
67,000
Ovalbumin,
43,000
Carbonic anhydrase,
30,000
Trypsin Inhibitor,
20,000
Part A
Describe the native protein, in terms of the kinds of subunits present, the stoichiometry of subunits, and the kinds of bonding (covalent,
noncovalent) existing between subunits.
Match the items in the left column to the appropriate blanks in the sentences on the right.
absence
ionic
four
Mw≈ 70,000 and
Mw≈ 40,000
covalently
hydrogen
two identical
Mw~ 70,000
Mw30,000 and
Mw≈ 40,000
noncovalently
presence
Mw≈ 70,000 and
Mw≈ 30,000
disulfide
There are
by the pattern of
The native molecule contains
the pattern of
The
in the
in the
linked subunits that are suggested
of BME.
Reset
units that are suggested by
of BME.
units are paired by at least one
Help
bond.
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