biochemistry question, please show all work! As the result of a storm while on a three-hour boat tour, you have found yourself on an uncharted island with several other people. The (additional) bad news is that you and your fellow castaways have become infected with a previously unknown, lethally pathogenic organism, P. Fortunately, however, you have discovered a biochemistry laboratory on the island, and you begin to study the organism. In Organism P, the ATP synthase enzyme complex can synthesize ATP using a proton gradient, and can use ATP hydrolysis to generate a proton gradient. For Organism P you measure: pHinside = 8.5, pHoutside = 7.3, and membrane potential = –110 mV. a. Assumingthatthebacteriumisaspherewithadiameterof2.34μm,howmany “free protons” are present in the cell? b. Assuming [ATP] = 5 mM, [ADP] = 1 mM, and [Pi] = 2 mM, and the cellular temperature is 25°C, what is the minimum number of protons that must enter the cell to yield enough energy to synthesize an ATP from an ADP. (Please show your calculations). c. Assume that the stoichiometry for ATP synthase is three protons per ATP. Under these conditions, is the ATP synthase synthesizing or hydrolyzing ATP? Please justify your answer.
biochemistry question, please show all work!
As the result of a storm while on a three-hour boat tour, you have found yourself on an uncharted island with several other people. The (additional) bad news is that you and your fellow castaways have become infected with a previously unknown, lethally pathogenic organism, P. Fortunately, however, you have discovered a biochemistry laboratory on the island, and you begin to study the organism.
In Organism P, the ATP synthase enzyme complex can synthesize ATP using a proton gradient, and can use ATP hydrolysis to generate a proton gradient. For Organism P you measure: pHinside = 8.5, pHoutside = 7.3, and membrane potential = –110 mV.
a. Assumingthatthebacteriumisaspherewithadiameterof2.34μm,howmany “free protons” are present in the cell?
b. Assuming [ATP] = 5 mM, [ADP] = 1 mM, and [Pi] = 2 mM, and the cellular temperature is 25°C, what is the minimum number of protons that must enter the cell to yield enough energy to synthesize an ATP from an ADP. (Please show your calculations).
c. Assume that the stoichiometry for ATP synthase is three protons per ATP. Under these conditions, is the ATP synthase synthesizing or hydrolyzing ATP? Please justify your answer.
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