2. ( Labeling molecules with isotopes has long been used to trace and characterize metabolic pathways. Upon oxidation in the mammalian liver of L- glutamate labeled as shown on the right, write the metabolic reactions that lead to the products as shown below using structure formulas. No need to include the enzymes. (a) succinate [ (1-14C)-succinate ] (b) (c) (d) citrulline [15NH2-CO-NH-(CH2)3-CH (NH3)-COO-] H 15 + 14! urea [ 15NH₂-CO-15NH₂] H-N H COO- aspartate [ (1-¹4C,2-15N)-L-aspartate and (4-14C,2-15N)-L-aspartate] C-H CH₂ L CH₂ ī COO- (2-14C,15N)-L-glutamate

2. ( Labeling molecules with isotopes has long been used to trace and characterize metabolic pathways. Upon oxidation in the mammalian liver of L- glutamate labeled as shown on the right, write the metabolic reactions that lead to the products as shown below using structure formulas. No need to include the enzymes. (a) succinate [ (1-14C)-succinate ] (b) (c) (d) citrulline [15NH2-CO-NH-(CH2)3-CH (NH3)-COO-] H 15 + 14! urea [ 15NH₂-CO-15NH₂] H-N H COO- aspartate [ (1-¹4C,2-15N)-L-aspartate and (4-14C,2-15N)-L-aspartate] C-H CH₂ L CH₂ ī COO- (2-14C,15N)-L-glutamate

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter19: The Tricarboxylic Acid Cycle

Section: Chapter Questions

Problem 4P: Understanding the Mechanism of the -Ketoglutarate Dehydrogenase Reaction tshe first step of the...

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