A biochemist is attempting to separate a DNA-binding protein (protein X) from other proteins in solutlon. On present. The proteins have the following properties. Protein pl 7.4 3.8 7.9 7.8 M.W. 82,000 21,500 23,500 22,500 DNA Binding? yes yes no yes What type of protein separation technique might she use to most effectively separate: a) protein X from protein A? b) protein X from protein B? E) protein X from protein C?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
icon
Concept explainers
Question
A biochemist is attempting to separate a DNA-binding protein (protein X) from other proteins in solutlon. Only three other proteins (A, B and C) are
present. The proteins have the following properties.
Protein
DNA Binding?
pl
7.4
3.8
7.9
7.8
M.W.
82,000
21,500
23,500
22,500
yes
yes
C
no
yes
What type of protein separation technique might she use to most effectively separate:
a) protein X from protein A?
b) protein X from protein B?
C) protein X from protein C?
Transcribed Image Text:A biochemist is attempting to separate a DNA-binding protein (protein X) from other proteins in solutlon. Only three other proteins (A, B and C) are present. The proteins have the following properties. Protein DNA Binding? pl 7.4 3.8 7.9 7.8 M.W. 82,000 21,500 23,500 22,500 yes yes C no yes What type of protein separation technique might she use to most effectively separate: a) protein X from protein A? b) protein X from protein B? C) protein X from protein C?
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Molecular techniques
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON