A DNA molecule that is 1000 base pairs in length would go molecule that is 200 base pairs in length. in the gel than a A) further B) you cannot tell from the information provided C) the same distance D) not as far

In DNA and plasmid extraction using the kit method, silica membrane is used. How could the nucleic acid attach to the membrane? O by phosphodiester bonding to the silica matrix O by hydrogen bonding in high salt conditions O by covalent bonding between nucleic acid and silica O by hydrophobic bonding in high chaotrophic salt conditions

It is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit will be used to isolate genomic DNA from this liquid culture. Answer the following questions to understand the strategy used by commercial kits for genomic DNA isolation. a)      List all the steps from cell pellet preparation to DNA elution. b)      With which feature can the membrane in the column that comes with the commercial kit bind DNA? c)      Which component in the kit would you use to recover the DNA from the membrane of the column to which the DNA was attached?

Which of the following statements regarding polynucleotides is true? a) A double stranded DNA forms a right-handed helix, where both strands run anti-parallel to each other. b) A double stranded DNA forms a left-handed helix, where both strands run anti-parallel to each other. c) A double stranded DNA forms a left-handed helix, where both strands run parallel to each other. d) A double stranded DNA forms a right-handed helix, where both strands run parallel to each other.

You have begun your career in medicinal biochemistry and have just discovered a bacterial DNA plasmld (transferabl ring of DNA) that appears to destroy the Ebola virus. In order to characterize your new plasmid, the molar mass of the plasmid must be determined. You dissolve 25.00 mg of the purified plasmid in 0.200 mL of water at 2 °C and find the osmotlc pressure of this solution is 1.20 Torr at 20 °C and 1 atm pressure. Answer the following about the Ebola-killing plasmid. 33.) The osmotlc pressure of the system is: (a) 1 atm (b) 0.016 atm (c) 6.5 X 10-5 atm (d) 22.59 atm (e) 0.0016 atm

For linear 34,000 kb DNA calculate a) contour length; b) the length of DNA as packaged in nucleosomes and c) the length of the DNA in a 30-nm fiber.

The words at the bottom with commas are the answer choices or word bank

ARE THEY TRUE OR FALSE? a) In a caesium chloride density gradient centrifugation method performed in the presence of ethidium bromide, supercoiled plasmid DNA binds more ethidium bromide than linear DNA. B)Tth DNA polymerase shows DNA-dependent DNA polymerase activity as well as RNA-dependent DNA polymerase activity. C)Magnesium ions stimulate polymerase activity. Therefore, it is better to use the highest concentration of magnesium in PCR amplification. D)When lambda bacteriophage infects E. coli cell lysis never occurs, and the infected bacterium can continue to grow and divide. E)The copy number refers to the number of molecules of an individual plasmid that are normally found in a single bacterial cell. F)RNase H selectively hydrolyzes phosphodiester bonds of RNA molecules in RNA:DNA duplexes.

What do you mean by amphoteric substance? Give examples of substance that is/are amphoteric. What is the purpose of the power supply in gel electrophoresis? Among the short and long strand of DNA, which moves the farthest in the gel and why? What is the purpose of  buffer in agarose gel electrophoresis?

For double-stranded DNA, which of the following statements is true? a) A = C b) A = G and C = T c) A + C = G + T d) A + T = G + C e) B and C are true