A researcher isolates a new protein and finds that it contains 0.755% serine by weight upon amino acid analysis. The molecular mass of serine is 105 g.mol-¹. What is the minimum molecular mass for the protein assuming only one Ser residue per protein molecule? Mmin = The researcher estimates the molecular mass of the protein using gel electrophoresis. From this estimated molecular mass, the researcher concludes the protein contains 3 Ser residues. What is the estimated molecular mass of the protein as shown by gel electrophoresis? g.mol-1 mest = g.mol-1

What is the minimum length of the column required to completely separate the amino acids? What is the time required for solvent elution (i.e. time for all amino acids to exit the column)?

Jacqueline isolated an interesting new protein from the broth of a yeast culture. After purification of the protein, she requested an amino acid analysis and found that the protein contained 0.57% tryptophan by weight (tryptophan Mr-204). Calculate the minimum molecular weight of the protein (i.e., assuming there is only one tryptophan residue per protein molecule).

A gel filtration column with a fractionation range of 1.5-20 kDa is used to separate out the proteins shown below. If these proteins are collected into separate fractions in between the void volume and total volume, in which order will they elute? Indicate if any of the proteins are found in the void volume or total volume fractions.                                                                        Protein Z - 3330Da                                                                        Protein Y - 13kDa                                                                        Protein X - 1.3kDa I. Total volume fraction II. Third protein fraction III. Second protein fraction IV. First protein fraction V. Void volume fraction