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- 1. A person ate 200 g of carbohydrates and then did not eat anything for a day. What process of carbon metabolism was stimulated in the liver 14 hours after the last meal? For answer: a) write a diagram of the process that speeds up in the liver after 14 hours after cating; b) indicate the regulatory reactions and their enzymcs; c) describe the mechanism of signal transduction by a hormone that regulates this metabolic pathway.5. Treatment of the patients with familial hypercholesterolemia by statins (pravastatin, for instants) allows to decrease cholesterol levels in blood up to the normal range. Explain the statin action on the cholesterol metabolism. For that: a) draw the scheme of the cholesterol synthesis, indicate the key enzyme of the pathway; b) name all mechanisms of the key enzyme regulation and statin's action; c) describe the structure of LDL receptor and its function in cholesterol metabolism; d) explain the cause of familial hypercholesterolemia and the symptoms of the disease.8. Patients on Irinotecan therapy increase production of hCES2 even though hCES2 breaks it down. (10.1093/inci/div132) Knowing this, How would you think about determining the optimum dosage of both drugs (Irinotecan and Compound 24) in a patient with chronic diarrhea/ulcerative colitis that is being treated for colon cancer? (There are a lot of right answers here). (Remember, Irinotecan and Compound 24 are BOTH binding hCES2).
- 2. After getting 300 g of carbohydrates with her meal, a student went to bed. What metabolic pathways of fatty acids have been activated in the liver about an hour after having a meal? For the answer: a) describe the glucose metabolism in the liver in this condition; b) name the metabolic pathway of fatty acids which has been activated; c) draw the scheme of this pathway and explain the fate of the end products of the pathway in the liver; d) explain the regulation of the pathway.1. What role do eicosanoids play in the body? What is the primary fatty acid in their composition? 2. What purpose does inflammation serve? What are some of the symptoms of inflammation?1. What is the significance of the effect of enzyme concentration to enzyme activity in relation to the medical aspect? a. A person with fever cannot eat well and may suffer from indigestion b. A person with hyperacidicity experience vomiting upon taking milk c. Abnormal level of diagnostic enzymes indicate damage in the body tissues d. Too much hydrogen peroxide in the body forms free radicals if not decomposed by peroxidase 2. What is the relation of the effect of temperature on enzyme activity to the medical field? a. To explain the cause why a feverish person cannot eat b. To explain why drinking carbonated leaves a fizzing sensation in our tongue c. To explain the cause why milk should not be taken by hyperacidic individual d. To explain why bubbles are formed when cleaning wounds with hydrogen peroxide 3. What reaction was involved in the determination of Vitamin C content of fruits? a. Oxidation…
- 5. A long-distance runner trained for a year to achieve better results. As he trained, he increased his mitochondrial capacity, as well as his oxygen delivery to the tissues. Why did these physiological mechanisms of adaptation to intensive physical activity allow him to win the competition? For the answer: a) name the hormone which controls the metabolism during physical activity and the fuel molecules secreted to blood from adipose tissue during prolonged physical activity; b) draw the scheme of their oxidation in the muscles; c) explain why the improved oxygen delivery to the tissues will affect this pathway.3. After 12 days of starvation, a man reduced his weight by 4 kg. The doctor prescribed him biochemical blood test to analyze the level of lipids in serum blood. The results showed an elevated level of free fatty acids and triacylglycerols. Explain the results of the biochemical blood test. For that: a) name the main hormone which manages metabolism during starvation; b) draw the scheme of this hormone action on the adipose tissue; c) name the possible pathways of fatty acids usage in the liver during starvation and write down the proper schemes.8. Hypercholesterolemia is a frequent complication of diabetes mellitus in patients with prolonged hyperglycemia. Why a high level of glucose in blood causes hypercholesterolemia and atherosclerosis? For the answer explain: a) how glucose can interact with the proteins and the consequences of this reaction for the proteins; b) glycation of which proteins results in hypercholesterolemia; c) possible causes and complications of hypercholesterolemia.
- A. How does each of the following irreversible inhibitors interact with the Enzymes? IV. 1. Cyanide 2. Heavy-metal ions B. List an antidote for each of these two poisons and describe how each functions.1. 65 years old woman with low physical activity got the excess carbohydrates (about 400 g daily) for several years. The laboratory value of total cholesterol in serum blood was 260 mg/dl. Why the excess consumption of carbohydrates can cause hypercholesterolemia? To answer the question: a) compare the cholesterol concentration in the serum blood of the patient with the normal range; b) draw the schemes of the metabolic pathways confirming that overeating of carbohydrates can result in hypercholesterolemia; c) draw the scheme of the cholesterol synthesis regulation; d) give your prescriptions for the diet and treatment of the patient.B) Read the situations below and indicate which of the four methods of enzyme regulation is occurring for each. a) The energy-carrying molecule ATP is made by the enzyme ATP synthase. Muscle cells use a lot of energy and also have higher amounts of the ATP synthase enzyme than many ouier cem types. General mechanism of enzyme regulation: /1 b) Prostaglandins are messenger molecules involved in the inflammatory response, as well as th perception of pain. They are synthesized from polyunsaturated fatty acid substrates by an enzyn called cyclo-oxygenase. "Ibuprofen" is the active ingredient in a variety of anti-inflammatory medications such as Motrin® and Advil®. It reduces pain and swelling by binding to a hydrophobic channel in the active site of cyclo-oxygenase, blocking the polyunsaturated fatty acids from binding to the enzyme, and therefore stopping production of prostaglandins. General mechanism of enzyme regulation: a) In point form, describe the steps by which ATP is produced…