83) The RDRP of the rotavirus a. Only produced in the first round of translation b. It is responsible to for the production of both the negative and positive sense strands of the dsRNA genome. c. Is working on the positive strand of the genomic RNA to produce the mRNA d. Is produced only in the second round of translation.

Someone submits a saliva sample for a PCR test to determine whether he is infected withSARS-CoV-2, an RNA virus. The first step is to purify RNA from the sample. Next, the RNA isconverted into cDNA.A. Why is this conversion important B. What is the first enzyme required for this step? C. Does this enzyme require a primer? Briefly explain. D. Three days later, the person submits another sample. The qPCR data againindicates a positive result; however, the spike gene Ct value is now 33. Based on the PCRdata, are they at the beginning or end of a COVID viral infection? Explain how you made thisdetermination

Alternative RNA splicing a. is a mechanism for increasing the rate of transcription. b. can allow the production of proteins of different sizes from a gene. C. can allow the production of similar proteins from different RNAS. d. increases the rate of transcription. e. is due to the presence or absence of particular snRNAs.

A sequence in the upstream leader of an mRNA that helps in identification of the start site of the open reading frame in bacteria. Group of answer choices a.Kozak Sequence b.Shine Delgarno Sequence c.Sigma Factor d.TATA Box