5. Using this formula Cu = Au x Cstd Astd Where: C = concentration A = absorbance U = unknown/sample STD = standard Compute for the glucose concentration of the patient's sample. Absorbance of the standard = 0.075; Concentration of the standard = 10.54mmol/L Absorbance = 0.83 mmol/L Convert your answer to mmol/L or S.I. using the conversion factor for glucose which is 0.055 Please show your computation.

a. calculate the volume of 0.1 M H2PO4– required to prepare 100 mL of your assigned buffer. b. calculate the volume  of 0.1 M HPO42– required to prepare 100 mL of your assigned buffer.  1. Assigned pH Value   ___6.90_________ 2. pKa Value of Assigned Buffer   ___7.20_________ 3. Ratio 0.5011

Determine the % inaccuracy by calculating the percent error of the micropipette at the set volume of    100 µL    A. Standard deviation  B. Covieffecrnt of varaiant C. % error (precision)   Percent error =  mean volume delivered  -expected volume  x  100                                                                         Expected volume       RUN Weight Measurement (in g) 1 0.0985 2 0.0987 3 0.0985 4 0.0982 5 0.0984 6 0.0984 7 0.0981 8 0.0983 9 0.0982 10 0.0988 Total 0.9841   A. Standard deviation  B. Covieffecrnt of varaiant C. % error (precision)

Calculate the Vmax with the parameters given. All parameters may not be needed to calculate. Kcat = 130 s^-1 Vo = 3.0 μMs^-1 S = 10 μM Et = 0.15 µM