Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Question
Plot based on chart
tube # mg protein/tube protein (4 mg/ml) 1 0x4=0 0 2 0-5x4=2 0.5 3 1.0×4 = 4.0 1.0 4 15x4l0 1.5 5 2.0x4=8-0 2.0 tube # ml albumin ml globulin ml 0.85% 6 7 8 o ml of stock human serum m1 of 0.85% saline 2.0 1.5 1.0 0.5 0 9 0.2 0.4 o✓ o✓ 0 0 1.0 2.0 saline Biuret 1.8 ✓ ✓ 1.6 ✓ 1.0 0 ✓ 3.0 0.10 3.0 0.25 30 0.01 3.0 0.02 ml Biuret 3.0 3.0 3.0 3.0 3.0 79.1% 56.9% 101-7% 104.8% A540 %T 0 0.11 0-18 0-31 0-38 A540 %T540 mg protein mg/ml protein in tube in tube 540 100% 77-6% 66-1% 48.5% 41-3%
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Transcribed Image Text:tube # mg protein/tube protein (4 mg/ml) 1 0x4=0 0 2 0-5x4=2 0.5 3 1.0×4 = 4.0 1.0 4 15x4l0 1.5 5 2.0x4=8-0 2.0 tube # ml albumin ml globulin ml 0.85% 6 7 8 o ml of stock human serum m1 of 0.85% saline 2.0 1.5 1.0 0.5 0 9 0.2 0.4 o✓ o✓ 0 0 1.0 2.0 saline Biuret 1.8 ✓ ✓ 1.6 ✓ 1.0 0 ✓ 3.0 0.10 3.0 0.25 30 0.01 3.0 0.02 ml Biuret 3.0 3.0 3.0 3.0 3.0 79.1% 56.9% 101-7% 104.8% A540 %T 0 0.11 0-18 0-31 0-38 A540 %T540 mg protein mg/ml protein in tube in tube 540 100% 77-6% 66-1% 48.5% 41-3%
paper, 2. Plot your standard curve (absorbance vs. concentration in mg protein/tube) on graph as in Fig. 5, and illustrate on your graph how the unknown concentrations (in mg protein/tube) are determined. Then calculate mg protein/ml protein by dividing the mg protein/tube by the m1 of albumin or globulin you added to each tube.
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Transcribed Image Text:paper, 2. Plot your standard curve (absorbance vs. concentration in mg protein/tube) on graph as in Fig. 5, and illustrate on your graph how the unknown concentrations (in mg protein/tube) are determined. Then calculate mg protein/ml protein by dividing the mg protein/tube by the m1 of albumin or globulin you added to each tube.
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