3. In vitro experiments are conducted at pH = 7.4 to simulate physiological conditions. A phosphate buffer system is often used. H₂PO H₂РO+H+ ркA = 7.2 a. What must be the ratio of the concentrations of HPO to H₂PO ions? b. What mass of NaH2PO4 must be added to 500.0 mL of 0.10 M Na₂HPO4 (aq) in the preparation of the buffered solution?

Human Anatomy & Physiology (11th Edition)
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please answer part a

3. In vitro experiments are conducted at pH = 7.4 to simulate physiological
conditions. A phosphate buffer system is often used.
H₂PO
H₂РO+H+ ркA = 7.2
a. What must be the ratio of the concentrations of HPO to H₂PO ions?
b. What mass of NaH2PO4 must be added to 500.0 mL of 0.10 M Na₂HPO4
(aq) in the preparation of the buffered solution?
Transcribed Image Text:3. In vitro experiments are conducted at pH = 7.4 to simulate physiological conditions. A phosphate buffer system is often used. H₂PO H₂РO+H+ ркA = 7.2 a. What must be the ratio of the concentrations of HPO to H₂PO ions? b. What mass of NaH2PO4 must be added to 500.0 mL of 0.10 M Na₂HPO4 (aq) in the preparation of the buffered solution?
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