40 mL of a 1: 8,000 dilution of the antibody in a single step

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You are tasked with measuring the quantity of prostate specific antigen (PSA) in a urine sample using an ELISA strategy. Describe and schematically depict how you would perform the test given the following materials: anti-PSA, biotinylated anti-PSA, streptavidin-HRP, HRP substrate kit (HRP = horse radish peroxidase).

please help me understand and answer this question on my study guide so that i can be prepared for my test!!

In the more common protocol for immunofluorescence detection of cellular proteins, an investigator uses two antibodies. The first binds specifically to the protein of interest. The second is labeled with fluorochromes for easy visualization, and it binds to the first antibody. In principle, one could simply label the first antibody and skip one step. Why use two successive antibodies?

You want to infect a plate of 2x10^6 HeLa cells with poliovirus at an MOI of 10. Your viral stock is at concentration of 8x10^7 particles/ml. What volume of your viral stock do you need to apply to your cells? Show your working

How would you prepare a 1:350 dilution of secondary antibody in a total volume of 3mL?

Condition; Acne Prone Skin Can we only use the facial gel A (facial gel A compose of water, Polysorbate 80, Propylene Glycol, Glycerin, Aloe Barbadensis Leaf Juice Powder, Carbomer, Hydroxypropyl Methylcellulose) wash the face to rinse off the sunscreen and any skincare in form of cream or serum, and does it enough if we use micellar water to clean the face first and then wash the face by using facial gel A for water-resistant sunsceen.

True or False: In complement fixation, a positive test result means that the red blood cells used in the test are destroyed

Answer is NOT D Help