Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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Concept explainers

Molecular Techniques
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DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological sampl…
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the fi…
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under …
Question
SDS is a __________ that gives proteins a _____________ charge while SDS-PAGE separates proteins based upon their ___________.
-chemical, negative, molecular mass
-chemical, negative, charge
-chemical, positive, charge
 
How would you prepare 40 mL of a 1: 8,000 dilution of the antibody in a single step?
a. 5 mL antibody added to 40 mL of TBST
b. 8 mL antibody added to 40 mL of TBST
c. 200μL antibody added to 40 mL of TBST
d. 5μL antibody added to 40 mL of TBST
 
On which molecule would you find an epitope?
-antigen
-antibody
-dna polymerase
-restriction enzyme
 

In the Western blot performed, which of the following proteins is recognized by the primary (1st) antibody?

a. BSA

b. goat anti-rabbit

c. rabbit anti-BSA

 

Insufficient washing of the membrane during the immunodetection will cause weak or no signal from the blot. 
TRUE OR FALSE
 

An agarose gel requires a specific orientation on the gel box in order to be poured.

TRUE OR FALSE

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