1 Apply the patient's blood sample to the antibody array. Covalently link antibodies to 2 each glycoprotein to a solid support. 3 Detect fluorescence. 4 Add fluorescently labeled lectins to the array. Each lectin should bind the glycan component of one glycoprotein under investigation.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Arrange the steps of performing the test to detect the presence of the glycoproteins indicating aggressive cancer in order, from start to finish.

1
Apply the patient's blood
sample to the antibody array.
Covalently link antibodies to
2
each glycoprotein to a solid
support.
3
Detect fluorescence.
4
Add fluorescently labeled
lectins to the array. Each lectin
should bind the glycan
component of one glycoprotein
under investigation.
Transcribed Image Text:1 Apply the patient's blood sample to the antibody array. Covalently link antibodies to 2 each glycoprotein to a solid support. 3 Detect fluorescence. 4 Add fluorescently labeled lectins to the array. Each lectin should bind the glycan component of one glycoprotein under investigation.
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