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- For the juice samples in exercise 3-4, you will be using the pour plate technique. In this exercise, you will add juice to a petri dish and pour agar over the sample and mix. Why is the media (potato dextrose agar, PDA) kept in a water bath until you are ready to use it, and why must you work fairly quickly? (One response answers both questions). The media is already solid and will form chunks The media has a dye added to it that must be kept at 45°C until ready to use O PDA is a media that allows growth of many types of fungi, and the heat from the water bath keeps the fungal spores from growing. The media is kept liquid in the water bath with heat, removing the tube from the heat source will cause the PDA to solidifyAnswer the following questionsExplain in your own words how the ISO 10993-10 test (performance test) is applied to the patient turning apparatus shown in the figure, and guess how the expected result will be. The main part of the product consists of a polyurethane sponge. Stain-proof upholstery fabric will be used as the outer surface material. In addition, there are heating pads working with the logic of electric blankets in this apparatus, please answer by taking these into account when answering.Answer this ff question: 1. Explain the importance of system suitability on HPLC method of analysis. 2.In UV and visible spectrophotometry, the specimen is generally dissolved in a solvent, and determinations are made at room temperature using a path length of 1 cm. Give the most common solvents suitable for UV or visible spectrophotometry.
- 3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTESShow all work including equations. Patient is treated AP/PA parallel-opposed fields. The fields are set up isocentrically on a 6 MV Linac, The patient's AP/PA separation is 25cm, and the field size at midplane is 14 x 26 cm (W x L). Calculate the AP field size on the skin.1.. What is the useful operating volume range of a Gilson P-10 pipette? Where did you find this information? 2.. Which micropipette should be used to dispense 4.50 x10-4 L of water? Explain your choice. 3. What should 2.50 x 10-4 L of water weigh at 27 °C? (Show calculations and include proper units with your answer) 4. Determine the temperature in the room where a gravimetric pipette calibration is being conducted. A 100 μl volume yielded a weight of 99.46 mg. Show your work for full credit.
- Describe what kind of pathological evalitations in short term in vivo test you would want to assess safety of new chemical intended for consumerExplain in your own words how the ISO 10993-5 test (performance test) is applied to the patient turning apparatus shown in the figure, and guess how the expected result will be. The main part of the product consists of a polyurethane sponge. Stain-proof upholstery fabric will be used as the outer surface material. In addition, there are heating pads working with the logic of electric blankets in this apparatus, please answer by taking these into account when answering.Someone want to you an antimicrobial agent that leads 99.999% inactivation when the initial microbial load was 10^7 CFU/mL.Firstly can you determine the experimental set-up. Clarify the dilutions you made and show the viable number of CFU from each dilution.
- 2. Compute for the calibration factor. Show your solution. CF = stage micrometer divisions subtended by om x value of one stage µm division ocular micrometer divisions subtended by stage micrometer (1 su x 10μm) 10 ou CF = CF = 1μm Ocular scale Stage micrometer scale or CF = Computation: (stage units x 10μm) ocular units 60 70 80 90 100 Name of Organism: Organism A Objective Used: HPO Total Magnification: 450x Ocular Micrometer units (violet rods): 10 Length of Organism:EXPERIMENT 2 Title: Aseptic technique Objectives: To apply the aseptic technique To observe the growth found on the petri dish. Material/apparatus: Gloves, incubator, soap for handwashing, tissues, nutrient agar, 70% alcohol Procedure: Wipe off the bench with 70% alcohol. Draw a line down the middle of the petri dish to divide the plate in half. Label each halves with A and B. Press your unwashed thumb onto the agar at column A. Apply proper handwashing technique. Put the same thumb after washing onto agar at the column B. Incubate the petri dish at 37°C for 24 hours or overnight. Observe and note down the colonies. Results:Question 4 (a) Microbiological contamination can be problematic for the pharmaceutical manufacturing industry. Describe BOTH strategies employed in the pharmaceutical industry to ensure acceptable microbiological stability. (b) Outline ANY FOUR specific sections that are clearly defined in stability testing protocols and name ANY TWO stability indicating analytical tests that are performed during stability testing. (c) Briefly discuss ANY TWO high stress/challenges that are utilised during an accelerated stability testing study. (d) Discuss how the shelf-life of a pharmaceutical drug formulation can be predicted.
