Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Transcribed Image Text:3. If PCR were performed on a highly conserved gene from two species, one prokaryotic
and one eukaryotic, would you anticipate different results or the same result? Why?
Transcribed Image Text:4. Using PCR, describe how you could determine if a strain of corn had a mutation
resulting in the partial deletion of a gene.
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A conserved gene is a gene that has remained largely unchanged over a long period of evolutionary time and is found in multiple species. This gene is typically important for basic cellular functions and plays a crucial role in the survival and reproduction of the organisms that carry it. The preservation of the gene sequence across different species is due to natural selection, which favors the preservation of functional genes over time. In molecular biology, conserved genes are often used as molecular markers to study evolutionary relationships, to identify and track the spread of diseases, and to develop new medical treatments..
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- What would happen if we use an endonuclease on DNA obtained from human cells(without PCR) & then electrophoresis it through a low-resolution gel.arrow_forward. In the PCR process, if we assume that each cycle takes5 minutes, how manyfold amplification would be accomplished in 1 hour?arrow_forward1) One of the first studies extracted all the DNA from a biofilm in an acid mine drainage in northern California in 2004. Then cloned the DNA in E. coli, sequenced all of the cloned fragments and then attempted to reconstruct the genomes of the different individuals in the population. This approach is an example of what? -Transcriptomics -Genomics -Metatranscriptomics -Metagenomics -Proteomicsarrow_forward
- why do you think a higher mortality rate ensures a large probability of getting mutantsarrow_forward67.arrow_forwardGriffith, in his 1928 experiments, demonstrated that bacterial strains could be genetically transformed. The evidence that DNA was the transforming principle responsible for this phenomenon came later. What was the key experiment that Avery, MacCleod, and McCarty performed to prove that DNA was responsible for the genetic change from rough cells into smooth cells? Pls help explain this question asap!!arrow_forward
- . Why do we need to amplify the DNA at all in order to visualize it in a gel? Why run PCR? . • Why do we need to include a search for the piece of DNA that codes for Tubulin if we are really only interested in finding, in this case, a commonly used GMO sequence? When we run that gel, we also run a ladder...why? What information does that give us? Why does it form a ladder shape on the gel (what is happening to cause things to separate out into many bands)? .arrow_forward1. Introduction: Define the following concepts and processes 1) List material used in a PCR reaction 2) List steps of PCR reaction and temperatures for each steparrow_forwardQ. How can you design your RT-PCR experiment to control for gDNA contamination? A. Use forward and reverse primers that bind to the same exon. B. Run a control lane where only RT was performed and not PCR. C. Run a control lane where mRNA has been amplified using PCR. D. Use forward and reverse primers that span the junction of 2 separate exons.arrow_forward
- 2. Please help answer this question and show all work, thank you so much.arrow_forward36. Which is the sequence of steps used to amplify DNA with PCR during a single cycle? 1) Primers bind (anneal) to complementary sequences of the target DNA. 2) DNA is heated to a high temperature to cause denaturation (DNA is single-stranded). 3) DNA polymerase extends the 3’OH of primers to make copies of the target DNA. A) 1, 2 and 3 B) 3, 2, and 1 C) 2, 1, and 3 D) 1, 3, and 2arrow_forward1. Primers come at a concentration of 200 pmol//ul (uM). You want the final concentration of your primers to be 0.2 uM. How would you dilute the primers in water so that you can add 1 ul in a PCR mix with a total volume of 50ul?arrow_forward
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