naterial used in a PCR reaction

DNA Extraction Using household materials Wash the fruit. Place the fruit (e.g. banana) in a zip lock plastic bag and crush it with your fist. Add the one half teaspoon of salt and one tablespoon of dishwashing liquid to the mashed fruit mixture in the bag, zip it up and squeeze it in your hands for 1 minute. Place the strainer over the small glass. Pour the mixture into the strainer. Filter the mixture into the glass. Slowly pour ice cold rubbing alcohol into the glass, until it is about half full. Let it stand until the alcohol will form a separate layer on top of the fruit filtrate. Keep the glass still at eye level; do not shake it. Watch what happens. DNA will begin to appear where t 9. Carefully Scoop out the DNA with the straightened end of the paper clip. Touch the DNA. he alcohol and filtrate layer meet A. In step 4, isopropyl alcohol is used to precipitate out the DNA because it is not soluble. What does this mean?  B. What role did the alcohol, detergent and salt play?…

Identify three items (aside from water) that must be used in a Polymerase Chain Reaction (PCR) mixture.

Explain how the spin columns were effective in collecting the isolated RNA from the sample and why adding water eluted the RNA into the tube for PCR diagnostic