3. Below is the result of a DNA (Sanger) sequencing reaction using the primer indicated on the right. A) Complete the DNA sequence by adding to the primer sequence below the image. Make sure you add the bases to the correct side of the primer! Dideoxy nucleotide used in sequencing reaction I largest smallest ddATP ddTTP ddCTP ddGTP - - 3'-AATTGCG-5' B) Now take the sequence you wrote above and make it double stranded (you don't need to include the primer). Label all 3' and 5' ends.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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3. Below is the result of a DNA (Sanger) sequencing reaction using the primer indicated on the right.
A) Complete the DNA sequence by adding to the primer sequence below the image. Make sure you
add the bases to the correct side of the primer!
Dideoxy nucleotide used
in sequencing reaction
largest
smallest
ddATP ddTTP ddCTP ddGTP
-
=
-
-
3'-AATTGCG-5'
B) Now take the sequence you wrote above and make it double stranded (you don't need to
include the primer). Label all 3' and 5' ends.
Transcribed Image Text:3. Below is the result of a DNA (Sanger) sequencing reaction using the primer indicated on the right. A) Complete the DNA sequence by adding to the primer sequence below the image. Make sure you add the bases to the correct side of the primer! Dideoxy nucleotide used in sequencing reaction largest smallest ddATP ddTTP ddCTP ddGTP - = - - 3'-AATTGCG-5' B) Now take the sequence you wrote above and make it double stranded (you don't need to include the primer). Label all 3' and 5' ends.
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