The temperature of hybridization (Th) is calculated using the Tm (melting temperature) of the dsDNA. This temperature is equal to: a. Th= 122 x Tm. b. Th 16.66 log Tm. c. Th=81.5 +0.41 (%GC) +16.6 log Tm. d. Th= Tm- (20-25°C). anded DNA

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