25. The restriction enzymes Kpnl and Acc651 recognize and cleave the same 6-bp sequence. You havea plasmid and a linear DNA strand that both contain a Kpnl and Acc651 sequence in the sameorientation as shown below. You digest both DNA pieces with both enzymes and then attempt toligate the sticky ends, followed by treatment with DNA ligase. What will happen?5' GGTACC3'5' GGTACC3'3 CCATGGS 3 CCATGGSKpnlAсс651A) You will produce sticky ends but the two types of ends will not ligate. Instead, you mayproduce a small amount of religated plasmid where the digested plasmid sequence re-inserts.B) You will produce a recombinant plasmid in which the linear DNA strand is ligated in betweenthe two sites, suitable for cloning.C) You will produce blunt ends that will not ligate because the two restriction enzymes will bothoperate on both of the sites.D) All the DNA will be completely digested as if you had applied a general DNAse enzyme.

Restriction enzymes are molecular scissors that are used in recombinant DNA technology to cut DNA at a specific site to produce restriction fragments. They are primarily a defense system against foreign pathogens.Answer: A) You will produce sticky ends but the two types of ends will not ligate. Instead, you may produce a small amount of religated plasmid where the digested plasmid sequence re-inserts. Explanation: A plasmid and a linear DNA strand digested with Konl and Acc651 restriction enzymes will yield sticky ends as follows: Kpnl 5' GGTAC........C 3' 3' C........CATGG 5' Acc651 5'G........GTACC 3'3 CCATG.........G 5' Both the restriction enzymes Konl and Acc651 recognize and cleave the same 6-bp sequence but they cut the sequence at different sites. So, it generates different sticky ends which will not ligate because they are not complementary to each other and do not fit. So the result is a religated plasmid or unwanted plasmid which prevents ligation of plasmid and DNA strands.