digest suspect B's DNA using restriction enzyme 1. Restriction enzyme 1 cleaves the recognition site 5' -AA/TT -3' . And count the fragment sizes from the digestion of the suspect.

Q: A. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the…

A: if we cut the plasmid with XhoI and XbaI then we will get three fragments of following sizes  1000…

Q: What is the principle of the Restriction Fragment Length Polymorphism (RFLP) in the diagnosis of…

A: Restriction fragment length polymorphism (RFLP) : it is a method used in genome mapping and…

Q: etion enzyme Sall cuts the sequence GTCGAC to leave a five base, 5' overhang. The restriction enzyme…

A: A restriction enzyme is classified under the category of a protein. These are isolated from bacteria…

Q: Describe how you can determine if the gene is interrupted, and if so the number of interruptions by…

A: Gel electrophoresis is a technique in which macromolecules such as DNA, RNA or proteins are broken…

Q: 1. Restriction enzymes can occasionally cut at site(s) other than its restriction site. 2. DNA…

A: NOTE:- As you have posted multiple questions, we will solve first two parts for you, To get the…

Q: A i. crossing over ii. biomarker a. Еcorl b. Recombinant iii. Gene-of-interest iv. restriction…

A: We know that crossing over is the major event during meiosis which leads to variations among…

Q: The other options are:   a. RNA cannot be digested by restriction enzymes b. RNA is small enough…

A: Northern blotting involve separation of RNA fragments by gel electrophoresis. In contrast Southern…

Q: Match the term with its description.

A: There are various enzymes and gene components that are studied in techniques of genetic engineering.…

Q: This is a restriction map for the 250 base pair plasmid pSage. Restriction sites for the restriction…

A: Restrictions endonucleases are enzymes that cut down the DNA into fragments near or at the site of…

Q: Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the…

A: The type of restriction enzymes that cut double stranded DNA at a particular spot is known as…

Q: Fill in the blank spaces by selecting the correct enzyme for each of the restriction enzyme…

A: DNA  is cleaved to form DNA fragments with the help of an enzyme known as the restriction enzyme.…

Q: The right-hand column contains descriptions of 5 types of molecular markers. Label each description…

A: These markers are pivotal tools within the field of genetics and genomics, helping researchers…

Q: How would the results of this activity have been different if the DNA sequence you digested were…

A: Gel electrophoresis is a process in which DNA fragments are separated on the basis of their size.…

Q: The restriction enzyme Xho I recognizes and cuts this sequence: 5-C/TCGAG-3. The restriction enzyme…

A: In molecular biology procedures, restriction enzymes are especially important for DNA cloning. They…

Q: Please answer both Part a. The plasmid you used as a template in your positive control for your PCR…

A: The Annealing Temperature is quite less in this case and hence there will not be proper or full…

Q: B) Suppose, you had a linear DNA instead of circular DNA. The double stranded sequence of a…

A: Introduction :- Restriction enzymes, also known as restriction endonucleases, are enzymes that can…

Q: Name the mapping technique used to determine the position of restriction sites in a DNA molecule.

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…

Q: Restriction digestion of DNA fragments is not sequence specific. True False

A: Within a few years after discovering EcoB, EcoK, and HindII, scientists were already experimenting…

Q: Write the 3' to 5' DNA bottom strand • Write the mRNA sequence of top strand Write the mRNA sequence…

A: In DNA   there are four nitrogen as base that is adenine, Thymine, guanine and cytosine is present.…

Q: In 5 sentences only, What are restriction enzymes (RE)? Describe how a RE can be used to…

A: Researchers continue to find new medications that aid in the treatment of deadly diseases and enable…

Q: Why does Northern blotting not require the use of restriction enzymes?

A: Restriction enzyme naturally cleaves DNA because it's have double stranded sequence of neucleiotides…

Q: Using the chart posted to the left of the microwave in lab, what is the 3. restriction recognition…

A: Step 1 In 1963, Arber discovered that Escherichia coli is able to protect itself from the attack of…

Q: Consider the following table of restriction enzyme recognition sites. The / indicates where the…

A: Introduction :-  Plasmids are small, circular, double-stranded DNA molecules that exist separately…

Q: Single and double digestion of plasmid pMCS326 were performed using the restriction enzymes AluIII…

A: Restriction mapping is a technique for mapping an unknown section of DNA by breaking it apart and…

Q: Select all the following statements that are correct regarding agarose gel electrophoresis and…

A: Agarose gel electrophoresis is a process used for separation of DNA fragments. Steps of agarose gel…

Q: dont provide handwriting solution ...

A: When dealing with circular DNA containing multiple EcoRI recognition sites, the digestion pattern…

Q: 30) In the CRISPR system, the targeted DNA sequence is

A: CRISPR stands for clustered, regularly interspaced short palindromic repeats. Engineered CRISPR…

Q: A restriction map lists the locations of DNA sequences that are cut by a particular restriction…

A: Restriction enzymes are found in bacteria as a defense against invading bacteriophages. Restriction…

Q: There are three classes of restriction enzymes; Class I, Class II and Class II. Nevertheless, Class…

A: Restriction endonuclease are the enzyme which make a cut in genetic material that is DNA somewhere…

Q: Using the plasmid map of pBCH2.0 provided above, predict how many DNA fragments would be formed if…

A: Restriction enzyme are specific DNA cutters and extremely useful while exploring molecular biology…

Q: Identify the basic steps (in right order) in AFLP marker analysis. Explain briefly how this marker…

A: Basic steps in AFLP marker analysis.

Q: Uncut - DNA Fragments produced B2 Sspl Enzyme(s) EcoR1 Smal used - 5.0 plasmid, how many times does…

A: Introduction :  The DNA-based structure known as a plasmid can exist and replicate on its own in…

Q: Which of the following is the DNA sequence that a restriction binds to?

A: DNA is the genetic element in all cell types of prokaryotic and eukaryotic. DNA is double-stranded…

Q: The following picture shows the ethidium bromide-stained bands obtained by gel electrophoresis of…

A: Electrophoresis is a molecular technique that employs the electrical field to assort biomolecules,…

Q: Why RT-PCR is important in the sample preparation to perform expression microarray experiment?

A: RT - PCR : Reverse transcription- polymerase chain reaction is one of the types of PCR.  DNA…

Q: molecular scissors", justify that

A: Restriction enzymes are found in a wide variety of prokaryotes. Restriction enzymes received their…

Q: ________________ "cut" DNA at specific sequences and the resulting segment with sticky ends is…

A: Recombinant DNA Technology is a kind of technology that utilizes certain enzymes to cut the DNA into…

Q: Above are the results of gel electrophoresis following digestion with restriction enzymes. What is…

A: Using specialized enzymes known as restriction endonucleases, restriction digestion is a laboratory…

Q: Restriction maps illustrate the lengths of DNA fragments between restriction sites. Which of the…

A: Restriction mapping is a technique to map unknown segment of DNA by breakung it into small…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.