2. What does an operon typically consist of? a. an operator, a promoter, and a cluster of genes b. a cluster of genes c. a promoter and a cluster of genes d. an operator and a cluster of genes e. a promoter and an operator

1. The lactose operon is controlled by both lactose and glucose. Fill in the following table to indicate what would be occurring under different cellular conditions Lactose absent present absent present Glucose Operon Activity (high/low) absent absent present present low high 10W high CAMP Repressor (bound/free) (high/low) bound free buind will high high low CAP (bound/free) bound free full

6. In the space below draw the lactose operon in an environment that LACKS lactose. On your image label the following items: a. DNA b. Lac promoter C. Lac Z gene d. Lac Y gene e. Lac repressor protein f. Lac I gene g. Lac operator h. RNA polymerase i. mRNA message (if made)

3 5. Suppose you performed a B-galactosidase assay on E. coli with a l*P+O°Z*Y operon. What results would you expect in the absence and presence of IPTG? 6. If you did not get the expected results for the previous question, but you knew the genotype of the operon was correct, name two ways your experiment could have gone wrong.