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Predict the level of genetic activity of the lac operon as well as the status of the lac repressor and the CAP protein under the cellular conditions listed in the following table (+ = sugar present; = sugar absent): Cellular conditions Lactose Glucose + + + a) Operon (on or off or basal?) b) Operon (on or off or basal?) Lac repressor (bound to operator?) CAP protein (bound to CAP site?) d) Operon (on or off or basal?) PROO Lac repressor (bound to operator?) CAP protein (bound to CAP site?) c) Operon (on or off or basal?) Lac repressor (bound to operator?) CAP protein (bound to CAP site?) Lac repressor (bound to operator?) CAP protein (bound to CAP site?)

Predict the level of genetic activity of the lac operon as well as the status of the lac repressor and the CAP protein under the cellular conditions listed in the following table (+ = sugar present; = sugar absent): Cellular conditions Lactose Glucose + + + a) Operon (on or off or basal?) b) Operon (on or off or basal?) Lac repressor (bound to operator?) CAP protein (bound to CAP site?) d) Operon (on or off or basal?) PROO Lac repressor (bound to operator?) CAP protein (bound to CAP site?) c) Operon (on or off or basal?) Lac repressor (bound to operator?) CAP protein (bound to CAP site?) Lac repressor (bound to operator?) CAP protein (bound to CAP site?)

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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**Predict the Level of Genetic Activity of the lac Operon** In this educational section, we aim to predict the level of genetic activity of the lac operon, along with the status of the lac repressor and the CAP protein under various cellular conditions. The table below indicates the presence (+) or absence (−) of lactose and glucose in the cellular environment: | Cellular Conditions | Lactose | Glucose | |:---------------------:|:--------:|:--------:| | a) | − | − | | b) | + | − | | c) | − | + | | d) | + | + | For each of the conditions (a, b, c, and d), we need to determine the following: - Whether the operon is on, off, or at a basal level of activity? - Is the lac repressor bound to the operator? - Is the CAP protein bound to the CAP site? ### Detailed Explanations: #### Condition a) - **Operon:** On or off or basal? - **Lac Repressor:** Bound to operator? - **CAP Protein:** Bound to CAP site? #### Condition b) - **Operon:** On or off or basal? - **Lac Repressor:** Bound to operator? - **CAP Protein:** Bound to CAP site? #### Condition c) - **Operon:** On or off or basal? - **Lac Repressor:** Bound to operator? - **CAP Protein:** Bound to CAP site? #### Condition d) - **Operon:** On or off or basal? - **Lac Repressor:** Bound to operator? - **CAP Protein:** Bound to CAP site? **Explanation:** 1. **Lactose (−) and Glucose (−)**: No lactose to induce the operon and low glucose leading to active CAP protein due to high cAMP levels. 2. **Lactose (+) and Glucose (−)**: Lactose present to induce the operon and absence of glucose will activate CAP protein. 3. **Lactose (−) and Glucose (+)**: No lactose to induce the operon and glucose presence will inactivate CAP protein. 4. **Lactose (+) and Glucose (+)**: Lactose present to induce the operon but high glucose will suppress CAP protein binding due to low cAMP levels.
Transcribed Image Text:**Predict the Level of Genetic Activity of the lac Operon** In this educational section, we aim to predict the level of genetic activity of the lac operon, along with the status of the lac repressor and the CAP protein under various cellular conditions. The table below indicates the presence (+) or absence (−) of lactose and glucose in the cellular environment: | Cellular Conditions | Lactose | Glucose | |:---------------------:|:--------:|:--------:| | a) | − | − | | b) | + | − | | c) | − | + | | d) | + | + | For each of the conditions (a, b, c, and d), we need to determine the following: - Whether the operon is on, off, or at a basal level of activity? - Is the lac repressor bound to the operator? - Is the CAP protein bound to the CAP site? ### Detailed Explanations: #### Condition a) - **Operon:** On or off or basal? - **Lac Repressor:** Bound to operator? - **CAP Protein:** Bound to CAP site? #### Condition b) - **Operon:** On or off or basal? - **Lac Repressor:** Bound to operator? - **CAP Protein:** Bound to CAP site? #### Condition c) - **Operon:** On or off or basal? - **Lac Repressor:** Bound to operator? - **CAP Protein:** Bound to CAP site? #### Condition d) - **Operon:** On or off or basal? - **Lac Repressor:** Bound to operator? - **CAP Protein:** Bound to CAP site? **Explanation:** 1. **Lactose (−) and Glucose (−)**: No lactose to induce the operon and low glucose leading to active CAP protein due to high cAMP levels. 2. **Lactose (+) and Glucose (−)**: Lactose present to induce the operon and absence of glucose will activate CAP protein. 3. **Lactose (−) and Glucose (+)**: No lactose to induce the operon and glucose presence will inactivate CAP protein. 4. **Lactose (+) and Glucose (+)**: Lactose present to induce the operon but high glucose will suppress CAP protein binding due to low cAMP levels.
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