2.) One experimental tool used in the biological research and in clinical settings is called Fluorescent Activated Cell Sorting (FACS) or cell cytometry. For example, a cell membrane permeable (or nonpolar) chemical called Hoescht 33342 dye will specifically label DNA. Interpret the graph (Figure 17-4) that illustrates normal cells that have been labeled with Hoescht 33342 dye. and assign which phase(s) of the cell cycle (G1, S, G2, or M) corresponds to the fluorescence intensity. Justify your answers. relative fluorescence per cell Figure 17-4 Analysis of Hoechst 33342 fluorescence in a population of cells sorted in a flow cytometer (Problem 17-15). number of cells

2.) One experimental tool used in the biological research and in clinical settings is called Fluorescent Activated Cell Sorting (FACS) or cell cytometry. For example, a cell membrane permeable (or nonpolar) chemical called Hoescht 33342 dye will specifically label DNA. Interpret the graph (Figure 17-4) that illustrates normal cells that have been labeled with Hoescht 33342 dye. and assign which phase(s) of the cell cycle (G1, S, G2, or M) corresponds to the fluorescence intensity. Justify your answers. relative fluorescence per cell Figure 17-4 Analysis of Hoechst 33342 fluorescence in a population of cells sorted in a flow cytometer (Problem 17-15). number of cells

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter10: Cell Division And Mitosis

Section: Chapter Questions

Problem 1ITD: Biologists have long been interested in the effects of radiation on cells. In one experiment,...

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In order to investigate the cell cycle in HEK293 cells in culture, I ran a flow cytometry experiment. I labeled their nuclei with DAPI to analyze DNA content. In these flow cytometry plots below, label the two main phases of the cell cycle that you can see. Briefly, explain your answer.
Suppose cells in an experiment had been labeled with green fluorescent tubulin. At the onset of Anaphase B, you use your laser to bleach a stripe across all of the microtubules on one side of the spindle as shown by the dashed line. This does not hurt the function of the microtubules in any way, but the bleached, nonfluorescent subunits in the microtubules now serve to mark a fixed location relative to the (+) and (-) ends. pl. membr. spindle A. Label one of each of the following: kinetochore MT, astral MT, polar MT. Indicate (+) and (-) ends. B. As the cell progresses through anaphase B, do the bleached spots get closer to, further from, or stay the same distance from the spindle pole they are embedded in? Why? Do they get closer to, further from, or stay the same distance from the plasma membrane? Why?
4) Describe in detail how p53 and MDM2 regulate cell division in a normal, healthy cell. You should describe 1) how these proteins cooperate to allow a cell to go through the cell cycle, 2) how they cooperate to stop the cell cycle, and 3) how they allow the cell cycle to continue again after having stopped it initially. You may use point form if you want.
1) Examine the picture of an onion root tip with cells in various stages of the cell cycle. A student claims that interphase is the longest stage of the cell cycle. Another student claims that mitosis is the longest stage. Using evidence from the photo, determine which student is correct and why. A) Mitosis is the longest stage because anaphase requires more time to separate chromatids. B) Interphase is the longest stage because chromosomes are visible in the majority of the cells. C) Mitosis is the longest stage because the majority of the cells in the photo are replicating their DNA. D) Interphase is the longest stage because the majority of the cells in the photo have uncondensed chromatin and are not dividing. Not Graded
Biologists have long been interested in the effects of radiation on cells. In one experiment, researchers examined the effect of radium on mitosis of chick embryo cells growing in culture. A population of experimental cells was examined under the microscope for the number of cells in telophase (as a measure of mitosis occurring) before, during, and after exposure to radium. The results are shown in the Figure. What is the effect of radium exposure on mitosis? Source: R. G. Canti and M. Donaldson. 1926. The effect of radium on mitosis in vitro. Proceedings of the Royal Society of London, Series B, Containing Papers of a Biological Character 100:413419.
Describe how the duplicated bacterial chromosomes are distributed into new daughter cells without the direction of the mitotic spindle.
Draw a diagram of the cell cycle and label the phases. On your diagram: a) For each phase, indicate whether the chromosomes are condensed or not. b) Indicate the phase(s) when it is possible to do a karyotype c) Indicate the phase-transition(s) where the cell would arrest if you add hydroxyurea.
3) Examine the graph showing the relative percentage normal and cancer cells spend in various stages of the cell cycle. Based on the information in the graphs, infer how cancer cells differ from typical, noncancerous cells. Select ALL that apply. A) Cancer cells do not replicate their DNA. B) Cancer cells replicate their DNA too quickly. C) Cancer cells do not go through interphase during their cell cycle. D) Cancer cells spend more time dividing compared to typical cells. E) Cancer cells do not always grow to the same size as typical cells. more than 1 answer. not graded
You are studying a new fungus, G. lucasus, and are interested the molecules that control the cell cycle of this new organism. You make some mutant cells and find an intriguing mutant that has two mutations - one in the force gene and one in the darkside gene. a. You perform propidium-iodide cell cycle analysis by flow cytometry on wild-type and the double mutant cells and your results are below. During what stage(s) of the cell cycle are the double mutant cells arresting their growth? Please briefly explain your answer. # of cells Wild-Type Cells In 180 kDa 110 kDa 80 kDa b. In studying the gene products of the force and darkside genes, you determine that the force gene produces a polypeptide of 80 kDa, and the darkside gene produces a polypeptide of 42 kDa. You collect lysates from wild-type cells in G₁, S, or G₂/M phases of the cell cycle and perform a Western blot (immunoblot) with either an antibody against force or an antibody against darkside ... only you forget to label your…
Hoechst 33258 is a chemical dye that binds specifically to DNA in live cells, and when excited by UV light, it fluoresces in the visible spectrum. Name and describe one specific method, employing Hoechst 33258, an investigator would use to isolate fibroblasts in the G2 phase of the cell cycle from those fibroblasts in interphase.
Explain why we can say that M-phase of the cell-cycle is triggered by a positive feedback loop. a) What would the consequences be if cohesins were working normally but condensins were not? and b) what stage of the cell cycle would this cause problems in? Why is it important for the centrosome to duplicate during G1-G2 (interphase) before M phase? The kinetochores serve as a link between the sister chromatids and the microtubules attached to the mitotic spindle. a) How are microtubules still able to exhibit dynamic instability after they are bound to the sister chromatids and b) why is this important to mitosis? As the name suggests, the Anaphase-promoting-complex (APC), promotes the 4th phase of mitosis by separating the sister chromatids so they can travel to separate poles of the cell, and prevents them from being re-zipped together. Describe how APC does these two things (Hint: one involves M-cyclin and the other involves…
1. (a) Describe the generalized model of the cell cycle. Demonstrate how cyclins and cyclin-dependent kinases can regulate the cell cycle. (b). Describe three ways in which cells lose their ability to regulate their growth to become cancerous.