Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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You have isolated a temperature-sensitive cell-cycle mutant. The mutated gene
encodes the activating subunit of the APC. The mutant protein is defective at high
temperatures. You sort several thousand cells and recover the WT-looking profile
below:
Cells (in thousands)
O G1
OM
O G2
OS
E
5
Next you grow the mutant at the restrictive high temperature and sort an equal
number of cells.
First, what phase of the cell cycle do you expect the cells to arrest in?
3
2
50
100
150 200⁰ 250
Propidium iodide fluorescence
♫
#tv
A X
MacBook Air
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Transcribed Image Text:You have isolated a temperature-sensitive cell-cycle mutant. The mutated gene encodes the activating subunit of the APC. The mutant protein is defective at high temperatures. You sort several thousand cells and recover the WT-looking profile below: Cells (in thousands) O G1 OM O G2 OS E 5 Next you grow the mutant at the restrictive high temperature and sort an equal number of cells. First, what phase of the cell cycle do you expect the cells to arrest in? 3 2 50 100 150 200⁰ 250 Propidium iodide fluorescence ♫ #tv A X MacBook Air
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