2. Draw a graph to calculate the initial rate of reaction due to enzyme X in absorbance units/min/100μl of extract.

Outline the steps involved in the two paths of sulfate assimilation.  Be sure to address the sulfur reduction pathway as a photoassimilation process.

2. The enzymatic reaction rate r is given by equation 2 below as a function of substrate concentration Cs and kinetics parameters KM and rmax. Using linear and nonlinear regression in excel, evaluate the kinetic parameter constants of a biosystem given the gathered data from a series of batch runs

1. How many mL of sterile water must be added to make the preparation isotonic? 2. How many mL of another isotonic solution must be added to make the final volume of the solution?

3. How would you use a 3-step serial dilution to produce 1ml of a luM Glucose solution from a 1M Glucose stock? What would be the total dilution factor?

SUBSTRATE CONCENTRATION [S] µM INITIAL VELOCITY V0    s-1 10 0.13 25 0.27 50 0.45 100 0.65 150 0.77 200 0.85 300 0.94 500 1.03 (i) a) Construct an empty table with the following column headings: Substrate concentration [S] and initial velocity (Vi) where [S] has the unit µM, and Vi has the unit mM/s. (ii) The table provided is the enzyme kinetic data for your mutated enzyme, whereby Vi was expressed using the unit ∆A(405 nm)/s. Using the standard curve, express Vi with the unit mM/s rather than ∆A(405 nm)/s. Place your answer in the table above alongside the appropriate [S]. Hint: To answer this question you need to use this standard curve equation=0.0419x (The slope of the line is= 0.0419) (iii) The unmutated form of your protein has a Km of 25 µM and a Vmax of 43 mM/s. The enzyme kinetic data for your enzyme with the amino acid substitution should now be displayed in the table above. Based on these data, what is Vmax? Km? and…

) The Chemical Solution lambda max Camax) ofa should be determined by absorbtion spectum 9) The standard curre Lambs Enzyme reaction_

● • 24) Determine the fraction of Vmax that would be found at a substrate concentration of ½2 Km, 2 Km and 10 Km.

1-. How would the extraction efficiency change if you changed the solvent from hexane to a more polar solvent like 1-chlorohexidine?  2-The cell wall of the microalgae is polar, can we use our method to extract lipids from microalgae?

Plot absorption versus time using the graph label mAbs vs time(s) from 0 to 120 s in 10-s intervals. Plot a line that can be used to calculate the initial rate using the graph label Initial Rate.