Based on the images attached, what does the similarity in the sequences tell you about the ermB gene found in these bacteria? Would we expect high or low sequence similarity when considering the possibility of horizontal gene transfer?

Your colleague wants to align the complete sequence of the collagen gene from humans to the homologous complete gene in rats. She can't decide if she should use the alignment program Needle or Water, and turns to you for help. Does it make a difference which program she uses, what advice would you give her?

This is about genetic of bacteria.

As mentioned in question 2 of More Genetic TIPS, origins oftransfer can be located in many different places on a bacterial chromosome,and their direction of transfer can be clockwise or counterclockwise.Let’s suppose a researcher conjugated six differentHfr strains that were thr + leu + tons str r azis lac + gal + pro + met +to an F − strain that was thr − leu − tonr str s azir lac − gal − pro −met −, and obtained the following results: Draw a map of the circular E. coli chromosome that shows thelocations and orientations of the origins of transfer in these six Hfrstrains.