
Biology 2e
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ISBN: 9781947172517
Author: Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher: OpenStax
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Transcribed Image Text:13.
For DNA, agarose is used as the main chemical to make the gel. What is the purpose of the buffer used on the gel?

Transcribed Image Text:16.
Which end of a primer does DNA polymerase add nucleotides to?
I.
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- DNA replication requires _______. a. DNA polymerase b. nucleotides c. primers d. all are requiredarrow_forwardChemical analysis shows that a nucleic acid sample contains A, U, C, and G. Is this DNA or RNA? Why?arrow_forwardIn DNA, nucleotide bonding forms a compound with a characteristic shape known as a(n) ________. beta chain pleated sheet alpha helix double helixarrow_forward
- DNA double helix does not have which of the following? antiparallel configuration complementary base pairing major and minor grooves uracilarrow_forwardHow many kilobases of the DNA strand below will code for the protein product?arrow_forwardWhich of the following phases is characterized by preparation for DNA synthesis? G0 G1 G2 Sarrow_forward
- Energy that drives the attachment of a nucleotide to the end of a growing strand of DNA comes from_______. a. ATP b. DNA polymerase c. the nucleotide d. a and carrow_forwardThe chain termination method of sequencing uses labeled ddNITPs uses only dideoxynucleotides uses only deoxynucleotides uses labeled dNTPsarrow_forwardUsing Figures 8.7 and 8.9 as a guide, draw a dinucleotide composed of C and A. Next to this, draw the complementary dinucleotide in an antiparallel fashion. Connect the dinucleotides with the appropriate hydrogen bonds. FIGURE 8.9 The two polynucleotide chains in DNA run in opposite directions. The left strand runs 5 to 3, and the right strand runs 3 to 5. The base sequences in each strand are complementary. An A in one strand pairs with a T in the other strand, and a C in one strand is paired with a G in the opposite strand. FIGURE 8.7 Nucleotides can be joined together to form chains caled polynucleotides. Polynucleotides are polar molecules with a 5 end (at the phosphate group) and a 3 end (at the sugar group). An RNA polynucleotide is shown at the left, and a DNA polynucleotide is shown at the right.arrow_forward
- Topoisomerases (a) synthesize DNA (b) synthesize RNA primers (c) join Okazaki fragments (d) break and rejoin DNA to reduce torsional strain (e) prevent single DNA strands from joining to form a double helixarrow_forwardWhen Griffith injected mice with a combination of live rough-strain and heat-killed smooth-strain pneumococci, he discovered that (a) the mice were unharmed (b) the dead mice contained living rough-strain bacteria (c) the dead mice contained living smooth-strain bacteria (d) DNA had been transferred from the smooth-strain bacteria to the mice (e) DNA had been transferred from the rough-strain bacteria to the smooth-strain bacteriaarrow_forwardThe statement DNA replicates by a semiconservative mechanism means that (a) only one DNA strand is copied (b) first one DNA strand is copied and then the other strand is copied (c) the two strands of a double helix have identical base sequences (d) some portions of a single DNA strand are old and other portions are newly synthesized (e) each double helix consists of one old and one newly synthesized strandarrow_forward
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