10. Like rotenone, cyanide is a broad-spectrum poison that affects many organisms. In fact, most poisons that act on the citric acid cycle, electron transport chain, and ATP synthase are broad- spectrum. What does this suggest about the evolution and conservation of these pathways?

5. Some enzymes can catalyze substrate reactions which result in the production of H* ions. If the batch reactor is not sufficiently buffered, this can lead to a change in pH. Because pH is an important factor in optimizing enzyme activity, the reaction velocity v can be altered during the reaction by the appearance of H* as a product. On a single graph, sketch a qualitative velocity profile (velocity vs. time) for the enzymatic breakdown of the substrate Auburn by the enzyme, WarEaglease for two conditions: A. An unbuffered solution that starts at pH 9. B. A well-buffered pH 9 solution. C. A well-buffered pH 7 solution. Relative Activity (%) 100 90 80 70 60 50 40 30 20 10 0 Activity of WarEagle ase 2 3 4 7 8 9 10 11 12 13 14 PH The substrate, Auburn, is a weak base, and the reaction is carried out in a batch reactor. Assume a high substrate concentration, so depletion of [S] does not cause the reaction to slow during the experiment. The relative activity of WarEaglease as a function…

10. In ATP synthase, the ____ subunit is the site of ATP synthesis while the ____ subunit forms the proton channel through the inner mitochondrial membrane.

The following image is a schematic of the metabolic processes occurring in a plant contained in a closed container.   A=Light Dependent reactions B=Glycolysis C=Light Independent Reactions D=Kreb's cycle E=electron transport chain   2a) Use the figure above to explain the conditions in which the oxygen concentration remains constant in the container. b) 2-carboxyarabinitol-1,5-bisphosphate (CABP) is an inhibitor of the enzyme rubisco. Which pathway would this affect most?