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Question:-
Biology
1. What is the difference between scanning and electron microscopy?
Step by step
Solved in 2 steps
- Q about fluorescence microscope: a. Summarize the mechanisms behind how fluorescence microscope work? b. provide a brief description on how a specimen would appear under a fluorescence microscope and whether or not the specimen can be living, dead, or both c. what is the fluorescence microscope most useful in viewing (what is the key use of fluorescence microscope)?PART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_ The field of view (FOV) is the circular area you can see when you look through the microscope. The diameter of the field of view is different depending on which objective lens you are using. For example, you are using the medium-power objective lens, then the area you can see is actually smaller than if you were using the low-power objective lens. Knowing The diameter of the field of view can help you estimate actual size of objects / cells seen through the microscope. When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1). Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the left edge of the field of view (see figure 2). NOTE: Slage cip I-1000 Objects in the FOV are usually measured in micrometers (um). To convert, a FOV in mm, times it by 1000…Analysis: Write your answers on the space provided. 1. How does the letter “e” as seen through the microscope differ from the way an “e” normally appears? 2. When you move the slide to the left, in what direction does the letter “e” appear to move? When you move it to the right? Up? Down? 3. How does the ink appear under the microscope compared to normal view? 4. Why does a specimen placed under the microscope have to be thin?
- topic: cheek cells 1. why are stains such as methylene blue used when observing cells under the microscope?Discussion Give the main differences between the types of microscopes used to diagnose surfaces?) What is the appropriate device to diagnose each of the following and why? O A- The aluminum plate is coated with a nano-solution. b- A colloidal sample containing nano-solutions. C- Study of the microstructure of the surface of a sample of iron.' Is it possible to use an optical microscope to study painted metal) surfaces? And why?Question:- Starting with data collection, describe the steps necessary to determine a 3-Dimensional structure using electron microscopy methods.
- Subject: Microbiology The author of a general biology textbook writes in reference to the development of antibiotic resistance, “The speed at which bacteria reproduce ensures that sooner or later a mutant bacterium will appear that is able to resist the poison.” How might this mutant bacterial cell appear? Do you agree with the statement? Does this bode ill for the future use of antibiotics?What is a microscope? What is its significance in the Medical Technology profession?Question:- What is the best superresolution microscopy method to resolve in z direction.
- 3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?You are in charge of buying new microscopes for your company, and you have been comparing different types of microscopes. One important property for microscopy is the resolution, which measures the ability to distinguish two small objects that are close together. Rank the following list of microscopes from lowest to highest resolution. Microscope List (5 items) (Drag and drop into the appropriate area) Transmission electron microscope (uses high energy electron beam) X-ray microscope (uses x-ray absorption to image sample, range 0.01-10 nm) Confocal fluorescence microscope (uses a blue laser as a light source, 488 nm) Level of Resolution 2 LowestHow to properly handle a Microscope? Explain. Thank you.