1. Draw a schematic of a stretch of DNA (horizontally) in the space below to represent a gene a. Put a green box around a section to indicate where the promoter would be b. Put an orange box around a section to indicate where the operator would be c. Put a blue box around a section to indicate where the RNA-coding sequences would be d. Put a red box around a section to indicate where the terminator would be

A sequence in the upstream leader of an mRNA that helps in identification of the start site of the open reading frame in bacteria. Group of answer choices a.Kozak Sequence b.Shine Delgarno Sequence c.Sigma Factor d.TATA Box

A small section of bacterial DNA template (anti-sense) strand has the following nucleotide sequence: AAG TAT TAT GCA A mutation in the above sequence involved the insertion of a single base, leading to a shift in the reading frame of the gene, resulting in altered amino acids downstream from the insertion. Which of the following gene sequences exemplifies the mutation described above? a. AAG CTA TTA TGC A b. AG TAT TAT GCA c. AAG TATU TAT GCA d. AAG TAT TAGT GCA

3. In future lectures we will describe a technique known as Northern blotting that can be used to detect RNA transcribed from a particular gene. Briefly, in this method a specific RNA is detected using a short segment of cloned DNA as a probe. The DNA probe, which is radioactive, is complementary to the RNA that the researcher wishes to detect. After the radioactive probe DNA basepairs to the RNA, the RNA is visualized as a dark (radioactive) band on an X-ray film. As shown here, the method of Northern blotting can be used to determine the amount of a particular RNA transcribed in a given cell type. If one type of cell produces twice as much of a particular mRNA as occurs in another cell, the band will appear twice as intense. Also, the method can distinguish if alternative RNA splicing has occurred to produce an RNA that has a different molecular mass. 875 nucleotides 675 nucleotides 1 Northern blot 2 - 3 Lane 1 is a sample of RNA isolated from nerve cells. Lane 2 is a sample of RNA…

Once bound to the promoter, RNA polymerase O a. melts the two DNA strands in the termination site region O b. degrades the two DNA strands in the start site region O C. separates the two DNA strands in the start site region O d. chemically alters the DNA template O e. breaks the DNA chain in the start site region

3. Inducers: a. transcribe a messenger off a DNA template b. bind to ribosomes to activate the production of specific proteins c. are a molecule that binds to a repressor to activate it d. are essential to function of transfer RNAs during translation e. bind to repressor protein and transcription of mRNA can then take place

Of the following, which is a modification made to the pre-mRNA of eukaryotes before translation?   a. The 5’ end receives a poly-A tail   b. All of the above   c. The 3’ end receives a cap   d. Introns are cut out and exons are spliced together