1. Briefly describe how you extract and purify the pJET plasmid from the bacteria.

explain F' plasmid transfer diagramatically

Please explain this picture about restriction enzymes

List the antibiotics that could successfully be used to test for the presence of the 4. gene on your plasmid.

1. A plasmid was digested with two restriction enzymes, individually and in a double digest. The following fragment sizes (bp) were determined by agarose gel electrophoresis. Restriction Enzyme|Fragment Size (bp) 30, 15, 5 HindIII 50 EcoRI + HindIII 20, 15, 10, 5 Make a restriction map of the plasmid based on this data and indicate the distances between the restriction sites.

i dont know what to draw

What is a plasmid?  How are they used in bioengineering? What are restriction enzymes? How do they work? What are restriction sites? What is the source of restriction enzymes? What is their function in nature?       4. Describe the function of electricity and the agarose gel in electrophoresis.

Describe the relevant features of the F factor (plasmid) that allow it to partake in conjugation.

What is the purpose of ampicillin in the pGLO lab, where is the ampicillin locates, how it can be described as selective, and how does it relate to the plasmid used?

describe F plasmid