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- Following is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300 Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial? Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…1. What is the importance of using freshly prepared KOH in testing the bacteria? Explain this comprehensively and please, do not just copy from somewhere.Please help a bit confused
- Which of the following does NOT describe a mechanism for drug resistance? Prevention of drug entry into the cell Production of proteins that bind and sequester the drug Creation of new plasmids upon drug treatment Changes in the structure of the drug's target Inactivation of the drug by hydrolysis. A second feature that virtually all plasmid vectors havein common is the selectable marker. Explain what this isand why it is such an important feature.A linear DNA fragment and a plasmid has three restriction sites for EcoRIhow many fragments will be produced from linear DNA and plasmid respectively.
- Recent scientific studies show that the plasmid plays a vital role in gene transfer not only in the field of bacteriology but microbiology on the whole. Give a detailed account of how bacterial gene transfer can be carried out as Medical Laboratory Scientist.During the incubation step of the plaque assay, viral replication leads to host cell lysis. This allows the freshly-released virions to diffuse through the media and infect new hosts. The amount of agar added to the top agar is 7 g per liter rather than the 16 g of agar per liter for most solid media, including the bottom agar used in this assay. How would it change the plaque assay results if bottom agar was used in place of the top agar in this assay? a- The diameter of the plaques would be smaller. b- The diameter of the plaques would not change. c- The diameter of the plaques would be larger. (2) A researcher measured the viral titer of PhiX174 in fish tank water, untreated sewage water, and unpasteurized apple juice. Which sample do you predict will have the highest titer on this page? a- Fish tank water b- Unpasteurized apple juice c- Untreated sewage waterYou first need to create a plasmid. What are the minimal components necessary to develop this plasmid? In addition to these components, please draw the plasmid. In this illustration, I am looking for the components, the direction of transcription (i.e., the direction the genes will be transcribed), and what should be transcribed last? Also, where would you specifically insert the P. falciparum gene in this plasmid, and why are you checking reading frames in your gene? Finally, please name your plasmid using the correct nomenclature too. You are excited you have this new plasmid; you want to transfect it into P. marinus and provide it as an oral vaccine to laboratory mice. However, even though your supervisor enjoys your enthusiastic attitude, they do not allow you to do this quite yet because all you have is this plasmid. Why doesn’t your supervisor allow you to use the laboratory mouse for this research regarding animal welfare guidelines? Your answer should include the 3 R’s of…
- Write a lab report on enumeration of lytic viruses : the plaque assay including the sections: introduction, Materials and Methods, Results and Discussion, and citing peer reviewed sources use this data for results section Group Number Number of Plaques Dilution Factor of Plate Original Concentration of Phage 1 58 10^3 5.80E+04 2 37 10^3 3.70E+04 3 44 10^3 4.40E+04 4 63 10^3 6.30E+04 5 36 10^2 3.60E+03 6 65 10^3 6.50E+04 7 260 10^2 2.60E+04 8 39 10^3 3.90E+04 9 178 10^2 1.78E+04 10 192 10^2 1.92E+04 11 274 10^2 2.74E+04 12 224 10^2 2.24E+04 Class Average 3.52E+04Need help To prove a BIM had evolved to be phage 2972 resistant, what experiment could you carry out? (already carried out 2% agarose gel PCR, what experiment could be carried out that is similar?)You perform a Kirby-Bauer assay with two antibiotics. Antibiotic X has a zone of inhibition of 9 mm. Antibiotic Y has a zone of inhibition of 11 mm. Which antibiotic is better at killing this particular microorganism? Group of answer choices 1Antibiotic Y 2Antibiotic X and Y, which have identical antimicrobial activities 3Antibiotic X4 4It is impossible to tell from the information given