2. How does the P-tube differ from the P+ tube? X P- has a control plasmid added to it that does not contain the gene for ampicillin resistance, while P+ contains a plasmid with the gene for ampicillin resistance. Feedback: Was a plasmid added to the P- tube during the experiment? P- contains the recombinant plasmid but no competent cells, while P+ contains both. Feedback: Was a plasmid added to the P- tube during the experiment?

2. How does the P-tube differ from the P+ tube? X P- has a control plasmid added to it that does not contain the gene for ampicillin resistance, while P+ contains a plasmid with the gene for ampicillin resistance. Feedback: Was a plasmid added to the P- tube during the experiment? P- contains the recombinant plasmid but no competent cells, while P+ contains both. Feedback: Was a plasmid added to the P- tube during the experiment?

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section: Chapter Questions

Problem 3TYK: Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning...

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Similar questions

Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.
A host cell has successfully taken up a recombinant pUC18 plasmid but the plasmid bears mutated origin of replication. Which of the following will happen to that host cell following amplification process on a media containing ampicillin and X-gal? * I It fails to form a colony on the media It survives in the media It would not be able to digest X-gal II III I and II only O I and III only Il and III only I, Il and III
1. Below is an illustration of two plasmids: the incomplete pKAN-R which contains the gene of interest (rfp), and the good plasmid vector pARA which contains the ampR gene for ampicillin resistance, the araC activator gene and the ori region. Based on this, how are you going assemble to create the desired recombinant plasmid containing all the necessary components? (Keywords: Identify correct restriction enzymes to use to create specific sticky end sequences that will only assemble with the final vector in one orientation)
3. Which of the following is not an important characteristic of a bacterial plasmid vector (shown)? (4) Plasmid pBR322 Host: E.com Ampicillin resistance- reporter gene lamp O Orgh of replication Hrdl +-Dants San Tetracycline rositance reporter gene (7) It should have the ability to integrate into the host's genome. It should have a reporter gene. It should have a gene for a selectable marker. O It should be small in size. O It should have the ability to amplify the gene being carried by producing many copies within the host cell. See Concept 13.2: DNA Can Genetically Transform Cells and Organisms
1. How large is your plasmid 2. Which colony(-ies) is(are) likely to have been white? 3. Which colony(-ies) is (are) likely to have the XRAY gene? asap please
3. A plasmid was digested with the enzymes BglII, Nsil and XhoI. A single digest was performed with each enzyme and then double digests were performed. Make a restriction map of the plasmid based on the data below and indicate the distances between the restriction sites Plasmid cut BglII Nsil Xhol Nsil + XhoI Nsil + BglII BglII + Xhol with: DNA| 33.2 31.2|47.8| fragments 18.2 20.2 3.6 (bp) 20.2 28.0 24.2 18.2 19.0 8.6 15.0 5.2 5.4 3.6 3.2 3.6
4. This is the set-up of our experiment. There are four plates shown: LB only (a type of nutrient agar), LB/AMP (LB agar with ampicillin included), and LB/AMP/Arabinose (LB agar with ampicillin and arabinose). All plates were spread with E. coli following the transformation procedure described in the video. Some of the E. coli samples were mixed with pGLO plasmid DNA (+DNA samples) and some E. coli samples were not (-DNA). Plate B -DNA Plate A LB LB/AMP Plate C Plate D +DNA LB/AMP LB/AMP/ARA 5. Complete the following chart to walk through the results you expect to see from the transformation procedure. 2 Based on B, IfE. coli coli added to coli ampicillin should the E. should grow, included as controls coli have grown on the glow? Did the E. Was the E. Plates A & B are the plate have resistant? the PGLO plasmid? (How do you plate? figure this out?) should it also for the lab. What do these plates tell you? (If no growth on plate A or growth on plate B) Plate A LB (general growth media)…
9. Here is a restriction map for a bacterial plasmid showing the cleavage sites for two different restriction enzymes EcoRI and Bam H1. EcoR1 700 bp EcoR1 2000 bp 1200 bp BamH1 Match the banding pattern in each lane of the agarose gel below with one of the treatments listed. Note: Not all treatments listed are run on the gel. Plasmid treatments: A. BamHI+EcoR1 (no RNAse) B. BamHI only (no RNAse) Gel lanes: 1. DNA Ladder = Mol. Wt. Standard (sizes indicated) bp 5000 4000 3000 2000 1650 1000 850 650 500 C. BamH1 + EcoR1 + RNAse D. RNAse only 2. E. BamH1 + RNAse F. EcoR1+ RNAse 3. G. EcoR1 only (no RNase) 4. 1 2 3 4 5 6 5. 6. -
3A. Following transformation with the CRISPR plasmid, the yeast will be plated on SD-URA plates. Why is SD-URA media used? Name the relevant marker gene in the pCRCT CRISPR plasmid. 3B. What would you expect to see if you plated on YED accidentally? Will most of the yeast be red or white? Why? 3C. The complete genotype of the host yeast strain is MATa ade2 his3 leu2 met15 ura3. Name one advantage of using a yeast strain with auxotrophies for several genes (i.e. his3, leu2 and ura3). 3D. After successful CRIPSR editing, the yeast can later be “cured” of the recombinant CRISPR plasmid – that is, the plasmid is lost but the CRISPR edit is stably inherited. Write the new genotype of the yeast-based on the ADE6 gene.
19. Which of the following statements is true for tumor inducing plasmids? Group of answer choices This plasmid can induce tumors in all plants The tumor kills the host organism soon after induction Their use provides an efficient method for expressing a eukaryotic gene in a prokaryote This plasmid comes from a eukaryotic organism The plasmid has a region used to produce opines where new genetic information can be added
1. Describe a certain species to use to for recombiant plasmids and explain what recombinant plasmids will do?
One of the frist steps in isolating plasmid DNA via mini-prep is to pellet the cells after O/N culture and then resuspend them in buffer P1. What's the point of pelleting the cells just to resuspend them again? To concentrate the cells P1 is a buffer, preventing small changes in pH O This step is not absolutely necessary P1 begins the lysis process D Question 2 What would happen if we didn't centrifuge the tube containing lysed cells after adding neutralization buffer and instead just added right to the column? O We could fihish the prep but we would have protein/RNA contaminants at the end O The precipitate is less dense than water so it shouldn't get in the way since it floats on top O The precipitate would obstruct the column and our prep is ruined O Our yield would be reduced, but we'd get something