1) Enhanced GFP protein has: An excitation peak at 488nm and emits maximally at 507nm. An excitation peak at 488nm and emits maximally at 587nm An excitation peak at 488nm and emits maximally at 567nm. An excitation peak at 458nm and emits maximally at 507nm.   2) A typical sequencing read will yield _____ nucleotides of unambiguous sequence. 1800-2000 3800-4000 2800-3000 800-1000   3) Sequencing primers should be designed to bind where? ~ 25 nucleotides upstream of the beginning region to be sequenced. ~ 25 nucleotides downstream of the beginning region to be sequenced ~ 60 nucleotides upstream of the beginning region to be sequenced. ~ 60 nucleotides downstream of the beginning region to be sequenced

Why do we need the KANR  gene next to the SPA tag sequence?  Briefly explain how and why it is going to be used in sORF function gene finding.

In Northern and Western blotting, what is the purpose of gel electrophoresis?

Utilizing Hind III and EcoR V Restriction Enzyme with Pet41 and the following gene of interest... a tgaaacaaca aaaacggctt tacgcccgat tgctgacgct gttatttgcg 61 ctcatcttct tgctgcctca ttctgcagca gcggcggcaa atcttaatgg gacgctgatg 121 cagtattttg aatggtacat gcccaatgac ggccaacatt ggaagcgttt gcaaaacgac 181 tcggcatatt tggctgaaca cggtattact gccgtctgga ttcccccggc atataaggga 241 acgagccaag cggatgtggg ctacggtgct tacgaccttt atgatttagg ggagtttcat 301 caaaaaggga cggttcggac aaagtacggc acaaaaggag agctgcaatc tgcgatcaaa 361 agtcttcatt cccgcgacat taacgtttac ggggatgtgg tcatcaacca caaaggcggc 421 gctgatgcga ccgaagatgt aaccgcggtt gaagtcgatc ccgctgaccg caaccgcgta 481 atttcaggag aacacctaat taaagcctgg acacattttc attttccggg gcgcggcagc 541 acatacagcg attttaaatg gcattggtac cattttgacg gaaccgattg ggacgagtcc 601 cgaaagctga accgcatcta taagtttcaa ggaaaggctt gggattggga agtttccaat 661 gaaaacggca actatgatta tttgatgtat gccgacatcg attatgacca tcctgatgtc 721 gcagcagaaa ttaagagatg gggcacttgg tatgccaatg aactgcaatt ggacggtttc 781…

Need help with understanding how to set up, pick and find reading frame for doing a gene insert...

Describe the purpose of each plate in an experiment.  Wild-type worms grown on the OP50-seeded plate Wild-type worms grown on the plate seeded with the pos-1 RNAi feeding strain egl-1 worms grown on the OP50-seeded plate egl-1 worms grown on a plate seeded with the pos-1 RNAifeeding strain

In the practical you have been analysing a human genomic library. You know from your calculations that only a small proportion of the human genome is represented, even when the entire class results are considered. Therefore, the chance of finding a particular single-copy gene in your library is very small. Outline a strategy for constructing a genomic DNA library more representative of the entire human genome. You will need to consider alternative vectors and the efficiency of transformation of the bacterial cells.