Q: Which is a limitation of using massively parallel sequencing ?
A: It is a term used to describe the method of high-throughput DNA sequencing to determine the entire…
Q: What are the five main steps in DNA cut-and-paste transposition?
A: The "cut and paste" method is used for saving conversion. The enzyme transposase acts as a pair of…
Q: What would be some major impediments to genetically modifyinghuman embryos with CRISPR?
A: Genome editing tools are those tools which help in making changes into the genome of an individual.…
Q: How to do linear Restriction Mapping of gene fragments, including a combination of three enzymes?
A: Bacteria contain restriction enzymes. To destroy viruses, bacteria use restriction enzymes, which…
Q: Name a special enzyme used by a "copy and paste" transposable element.
A: Transposable elements Transposable elements are sequence found in DNA that have ability to move…
Q: Why is it difficult in a single experiment to transfer a largenumber of genes to a recipient cell…
A: Gene transfer is the process of insertion of unrelated DNA into cells. There are three mechanisms of…
Q: What is the useful of the Development of highly sensitive and low-cost DNA agarose gel…
A: In today's medicine, extremely sensitive and low-cost DNA agarose gel electrophoresis is widely used…
Q: Describe step 1: hybridization of template and primer in automated sanger sequencing
A: Introduction Sanger sequencing is also known as the “chain termination method”. It is a method for…
Q: What are the advantages of using a restriction enzyme whose recognitionsite is relatively rare? When…
A: Restriction enzymes or restriction endonucleases are enzymes capable of recognizing a specific…
Q: What does CRISPR-Cas stand for and how does it work? What are the molecules involved with CRISPR-Cas
A: Introduction Genome editing:- It is also known as gene editing or genome engineering. It is a type…
Q: What is the significance of palindromic Restriction Endonuclease recognition and sequence overhangs…
A: Restriction endonucleases enzyme is also known as molecular scissors, which helps in cleavage of DNA…
Q: What are the possible bioethical issues that gene editing tools may encounter?
A: Gene editing tools are used to edit an organisms genetic code , i.e.. with these tools ,the genetic…
Q: What are three benefits Ion torrent sequencing has over sanger sequencing
A: DNA is an important biomolecule in the cell as it is the genetic material for most of the organisms…
Q: What is CRISPR-Cas9? What is one advantage and one disadvantage of this technology?
A: Gene editing is a technology in which Genetic material of an organism is altered and remodelled.…
Q: What are the vectors used in rDNA technology? briefly explain each
A: rDNA technology or Recombinant DNA technology is a widely used technology with the advancement of…
Q: What is Western blotting ?
A: Blotting refers to a technique by which a biomolecule is transferred from one medium to another…
Q: What is the difference between using genome build hg18 and hg19 in Genome-wide SNP arrays?
A: SNP (Single nucleotide protein) array is a type of DNA (deoxyribonucleic acid) microarray which is…
Q: What is it about the Cas9 protein that makes it an efficient DNAediting tool?
A: DNA and RNA are the genetic elements present in almost all cells. DNA encodes for proteins through…
Q: Which vectors can be used to clone a continuous fragment of DNA with the following lengths? a. 4 kb…
A: According to the question, we have to mention the name of the vectors that can be used to clone a…
Q: What is linear amplification PCR?
A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…
Q: How can linkage disequilibrium mapping sometimes provide a much higher resolution of gene location…
A: Linkage analysis rely on co inheritance of functional polymorphism, In linkage analysis, there are…
Q: Distinguish between a Northern, Southern and Western blot
A: Southern blotting is a blotting process for detecting particular DNA sequences, Northern blotting is…
Q: What is restriction fragment length polymorphism and which method do we use to detect it?
A: Restrictions length polymorphism (RFLP) is a kind of polymorphism that outcomes from variety in the…
Q: What are the advantages of Next Generation Sequencing?
A: DNA sequencing is a biochemical method for determining the order of nucleotide bases, A, G, C, and…
Q: Short tandem repeats (STR) profiling is based on
A: Small tandem repeats (STRs) are short repeating DNA sequences (2–6 bp) that make up roughly 3% of…
Q: What is the role of the following molecules in the CRISPR mechanism? *
A: CRISPR stands for clustered regularly interspaced short palindromic repeats. These are a set of DNA…
Q: Why is it necessary to boil the probe before you use it in your southern blot?
A: Southern blotting is a molecular biology technique that is used to detect a person's DNA. This…
Q: Give a step by step method and diagram of how reversible terminator sequencing works.
A: The reversible terminator sequencing or Illumina dye sequencing is a technique used to determine the…
Q: CPT code assignment 44970
A: Cpt code is current procedural terminology. These are the numerical codes helps to identify the…
Q: Why does reversible chain termination sequencing yield short reads?
A: In genetics, sequencing is considered a methodology to determine the order of nucleotides(adenine,…
Q: Briefly Explain different applications of western blotting. Please explain at your own words.
A: A western blot is an analytical molecular biology laboratory method that is used in the detection of…
Q: Gel Electrophoresis Separates DNAFragments According to which thing?
A: Electrophoresis is a technique used for separating the components of a mixture of charged molecules…
Q: What is an advantage of RNA Seq over microarrays?
A: The process in which the sequencing of total messenger ribonucleic acid (mRNA) is carried out…
Q: What is CRISPR?
A: Introduction Bacteria and viruses are tough competitor to each other, in order to overcome each…
Q: What is the difference between a map-based approach to sequencing a whole genome and a whole-genome…
A: Deoxyribonucleic acid (DNA) is a double stranded helical genetic material containing thousands of…
Q: What is the major difference between the strategies of map based sequencing and shotgun sequencing?
A: DNA sequencing is a process in which sequence(order) of nucleotides in DNA is determined. It…
Q: What are frame-shift insertion?
A: Mutations are changes that occurs in the deoxyribonucleic acid (DNA) sequence, either due to…
Q: What is Northern blot analysis ?
A: The nucleic acids transfer on a solid phase membrane for identification is called the blotting…
Q: Describe the features of a typical CRISPR locus in a bacterial cell.
A: CRISPR (short for clustered regularly interspaced short palindromic repeat) acts as the immune…
Q: How else can it be proven that transformants have the topA-cysB fragment apart from size analysis…
A: A transformant cell is a cell that has received additional genetic material, either experimentally…
Q: What is the difference between bulk RNAseq and single cell RNA seq?
A: In RNA sequencing, RNA is taken out from tissues of different cell types for analysis of RNA seq .…
Q: what are the advantages and disadvantages of CRISPR cas9 technology?
A: CRISPR -Clustered Regularly Interspersed Short Palindromic Repeats The CRISPR cas9 is a technology…
Q: What are the main steps of Western Blotting?
A: The technique of Western blotting is a well-known and commonly used method for detecting and…
Q: CRISPR
A: Interestingly, CRISPR-Cas9 could be used to the investigation of treatments of various human…
Q: what is the purpose of calcium chloride in the transformation process
A: Calcium chloride transformation is a laboratory technique in prokaryotic (bacterial) cell biology.…
Q: For the hybridization step in the Southern blotting, the hybridization was done at 85 degrees…
A: Southern blotting is the most common molecular biology technique. A Southern blot is defined as a…
In Northern and Western blotting, what is the purpose of gel electrophoresis?
Step by step
Solved in 2 steps
- Why are there multiple results returned when you input a sequence into BLAT or BLAST? How do you know which is most relevant? Does that make the others irrelevant?The best molecular technique to quantify the gene transcripts is (write in full).What needs to happen so the reporter molecule can emit fluorescence that is not quenched?