The purpose of this lab was to observe the rate of photosynthesis under different light conditions. Small circles were hole punched out of the spinach leaves. Then a 0.2% solution of sodium bicarbonate was prepared. There were three cups with the solution along with ten leaf disks per cup. The cups were exposed to light for an hour and the number of floating disk was recorded.
Introduction
Photosynthesis is the process through which plants use water and carbon dioxide to create their food, as well as to grow and release excess oxygen into the air. The purpose of photosynthesis is to convert water and carbon dioxide to glucose. Photosynthesis has two processes, each with multiple steps. The light reactions process splits water, releases oxygen, reduces the electron acceptor, and generates ATP from ADP. While the Calvin cycle forms sugar from oxygen using ATP and NADPH. Organisms like plants, alga, cyanobacteria, and purple sulfur bacteria undergoes photosynthesis. It is important for photosynthesis to occur because all living things needs oxygen to survive and photosynthesis gives us that
…show more content…
The procedure for this lab was to punch 30 uniform leaf disks in several spinach leaves, label three plastic cups with: Light, Ambient Light, and Dark. Then prepare a 0.2% solution of sodium bicarbonate and water in a larger beaker and add 2 drops of liquid soap to the solution. Remove the plunger from the syringe and place 10 leaf disk into the syringe. Insert the tip of the syringe into the solution and draw 15-20mL into the syringe. Then seal the tip of the syringe using your index finger and the leaf disk should began to sink. Repeat steps and place each solution into a cup, record the number of floating disks every five minutes for an
The purpose of this lab is to determine the relationship between photosynthesis and cellular respiration.The effect of Light Intensity experiment will show the rate of photosynthesis based on the amount of light from the light bulb, temperature, and direction and distance of the light, these variables determine the absorbance. In the effect of Light Wavelength experiment, photosynthesis is affected by different light colors. Photosynthesis in this experiment is more successful with certain colors due to different pigments in chloroplasts only absorbs certain wavelengths. The rate of photosynthesis will be estimating oxygen production in spinach leaf using floating leaf disk procedure. The more floating disks, the more oxygen being produces
The purpose of this lab is to understand the process of photosynthesis and how sunlight effect has on it. To prove that in order for photosynthesis to happen light is needed, and to see if temperature has an effect on how fast or how slow photosynthesis happens.
Purpose: In doing this lab, the objective was to find how the intensity of light effects the of the products of photosynthesis. This lab will measure to what extent production of photosynthesis increases when light intensity is increased. This experiment will not only provide a visual representation of the process, but will also allow for further examination of the relationship between photosynthesis and cellular respiration. Introduction: The process of photosynthesis uses energy from the sun to produce glucose for plants, and cellular respiration takes glucose and oxygen to form carbon dioxide and water.
Photosynthesis is the process plants use to make glucose and oxygen by using carbon dioxide from the atmosphere, water from underground, and the energy of sunlight. Photosynthesis occurs in the leaves of the plant because the cells there have organelles called chloroplasts. The chloroplasts contain chlorophyll, a chemical that absorbs the energy of sunlight. The light energy, the CO2 and the water that the plant has absorbed in its roots is converted to glucose and oxygen. The plant stores the glucose as starch for energy, and expels the oxygen through its leaves.
Photosynthesis is a key process for all life on earth and therefore determining the factors that affect photosynthesis can be very beneficial. It is the process by which compounds such as water and carbon dioxide, through light energy are converted into a more useful compound such as carbohydrate (Rush 2015). This process not only is beneficial for plants but is also helpful for humans as photosynthesis releases a key element that is
This experiment demonstrates the effects of pH on the rate of photosynthesis by examining the behavior of leaf disks in different pH solutions under light. In this experiment, we used five different pH levels: pH 5, pH 6, pH 7, pH 8 and pH 9. These solutions were created using a combination of hydrochloric acid and sodium hydroxide. Spinancia olcerea or spinach, leaves were used in the experiment to examine the effects of pH on the rate of photosynthesis. The rate of photosynthesis was measured by counting the number of leaf disks that rose to the surface of the solution after each minute. In acidic solutions, the rate of photosynthesis increased while in basic solutions, the rate of photosynthesis decreased.
The leaf with the highest rate of photosynthesis was the darkest leaf, the purple cabbage. The leaf with the lowest rate of photosynthesis was the lightest leaf, the iceberg lettuce. We can conclude that the rate of photosynthesis was much higher in the purple cabbage because darker leaves contain more chloroplasts. Therefore, photosynthesis occurs much more quickly. Notably, these results support the stated hypothesis. An error that might have affected the data as a whole was the fact that the calculated rate of photosynthesis for the iceberg lettuce was negative. In addition to this, a few of the other percentages decreased as time passed. This means that the chloroplasts would have been going through cellular respiration. This inconsistency might have transpired from a mechanical error within the spectrophotometer. The control tubes, the “boiled” and “dark” tubes, of all 3 leaves were necessary in order to determine if the chloroplasts were the organelles of the cell that were causing one leaf to photosynthesize quicker than another because it was imperative for the chloroplasts to be exposed to light and temperatures that would not denature them. Therefore, we are able to conclude that the chloroplasts located within the leaves were the organelles that were causing the varying rates of photosynthesis in this
The results from this experiment concludes that the yellow plastic film over the solution containing spinach leaf disks increased the rate of photosynthesis the most. This outcome rejects the initial hypothesis that the red film would increase the rate of photosynthesis the greatest, which may be due to the fact that the yellow film allowed the most light to travel to the leaves. This experiment then, experimented on the effects of light intensity on the rate of photosynthesis rather than the outcome of how differing wavelengths affect photosynthesis rates. Thus the reason why the yellow film treatment increased the rate of photosynthesis most is due to the fact that as more photons of light that reach the leaf, the greater the number of chlorophyll
After analyzing the results of the paper chromatography, the next step was the determination of the absorption spectrum for the spinach leaf pigments chlorophyll a, chlorophyll b, xanthophyll, and carotenoids. The amount of absorbance was determined with the help of the spectrophotometry, which quantitatively measured the fraction of the light passing through each pigment extract solution, indicated on the absorbance scale. The wavelength was increased every 20-nm, starting with 400-nm, and reaching 740-nm. The results are shown in Table 1 and Table
In photosynthesis, there are two types of reactions; one is light dependent and second one is light independent. During the light reaction, the light energy from sun is converted to chemical energy in the form of ATP and NADPH. This chemical energy is then used in second light-independent reaction to convert atmospheric CO2 to sugar and this process is also known as carbon fixation. The second cycle that occurs in absence of light is also called Calvin cycle. Along with sugar molecule, O2 is released as a by-product.
In photosynthesis, there are two main parts, including light dependent and light-independent reactions. Plants use the energy from light for producing sugar, which is being converted into ATP by cellular respiration. They also are the only organisms that produce oxygen along with glucose and fructose chains within the light-independent phases of photosynthesis. This process takes place in the chloroplasts of plants, which include chlorophyll. Chlorophyll is essential for photosynthesis, since it absorbs the sun’s light. Green
Photosynthesis is a vital process in plants as carbon dioxide is used to form sugars and other complex compounds for growth. There are two basic processes in photosynthesis: light-dependent and light-independent.
Photosynthesis is the process undergone by plants consisting of a series of chemical reactions that require four factors; water, carbon dioxide, radiant energy and temperature using chlorophyll molecules to trap radiant energy from the sun resulting in the making of glucose and oxygen as bi-products. The process of photosynthesis provides plants with the food and energy they need to grow. Plants that produce their own food are called autotrophic plants. Not only do these plants make food for themselves but they also serve as primary producers. Without photosynthesis the oxygen levels in the atmosphere would not be replenished. The process of photosynthesis is divided into two main parts. The first phase is the light dependent phase which
Photosynthesis is a biochemical process in which plant, algae, and some bacteria harness the energy of light to produce food. Nearly all living things depend on energy produced from photosynthesis for their nourishment, making it vital to life on Earth. It is also responsible for producing the oxygen that makes up a large portion of the Earth¡¦s atmosphere. Factors that affect photosynthesis are light intensity and wave length, carbon dioxide concentration, and temperature.
In this project I believe the outcome will be that light energy is essential for photosynthesis to occur. The aim was to show that light is necessary for photosynthesis. Photosynthesis is the process of converting light energy into chemical potential energy and storing it as starch. This process occurs in plants and some algae. Plants need light energy, CO2, and H2O to make sugar. The process of photosynthesis takes place in the chloroplasts,