I. Introduction
Photosynthesis is the process by which plants use light to synthesize food from H2O and CO2 within the thylakoid and the stroma of the cells. In order for photosynthesis to occur, light must be present. Light reactions occur within the thylakoids of the chloroplasts by absorbing light and H2O and producing oxygen gas, ATP, or Adenosine triphosphate, and NADPH, or nicotinamide adenine dinucleotide phosphate. The oxygen gas is released back into the atmosphere while ATP and NADPH are inputs of the Calvin Cycle within the stroma. The Calvin Cycle uses these two molecules, in addition to carbon dioxide gas, to produce ADP, or Adenosine diphosphate, NADP+, and glucose molecules. Photosynthesis is represented by the following equation:
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It showed that there was no CO2 present for the input of the photosynthesis reaction so the oxygen given off must have come from the H2O. This means that since photosynthetic reactions occurred without CO2 present, light reactions did not necessarily need CO2 to occur. The reaction only needed water and light. Oxidation-reduction reactions are initiated by light and are significant in photosynthetic reactions. When light reactions occur, electrons reduce NADP+ by adding an H+ ion, which forms NADPH. DPIP is a blue dye that is used to detect photosynthetic reaction rates by intercepting these electrons that flow during photosynthesis. The transition from blue dye to colorless signifies that photosynthesis is occurring. This reaction is represented by the following …show more content…
The leaf with the highest rate of photosynthesis was the darkest leaf, the purple cabbage. The leaf with the lowest rate of photosynthesis was the lightest leaf, the iceberg lettuce. We can conclude that the rate of photosynthesis was much higher in the purple cabbage because darker leaves contain more chloroplasts. Therefore, photosynthesis occurs much more quickly. Notably, these results support the stated hypothesis. An error that might have affected the data as a whole was the fact that the calculated rate of photosynthesis for the iceberg lettuce was negative. In addition to this, a few of the other percentages decreased as time passed. This means that the chloroplasts would have been going through cellular respiration. This inconsistency might have transpired from a mechanical error within the spectrophotometer. The control tubes, the “boiled” and “dark” tubes, of all 3 leaves were necessary in order to determine if the chloroplasts were the organelles of the cell that were causing one leaf to photosynthesize quicker than another because it was imperative for the chloroplasts to be exposed to light and temperatures that would not denature them. Therefore, we are able to conclude that the chloroplasts located within the leaves were the organelles that were causing the varying rates of photosynthesis in this
Light intensity is a key component in photosynthesis, amongst carbon dioxide and water to sustain a suitable rate of photosynthesis. Chlorophyll absorbs the light, causing photoexcitation and the formation of NADPH and ATP with production of O2 as a by-product. The Calvin Cycle takes the NADPH and ATP to reduce CO2 into sugars (CH2O), and return NADP+ and ADP + Pi to the light reactions. The process will then repeat. (Reece, et al, 2015)
Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.
Photosynthesis is the process in which plants consumed inorganic materials like solar light, carbon dioxide and water and converted it to an organic molecule like sugar and an inorganic gas like oxygen. Light is one of the major elements influencing the rate of photosynthesis; direct light concentration affects the noncyclic pathway (light
Photosynthesis is a process used by plants and other organisms to convert light energy into chemical energy that can later be released to fuel the organisms ' activities. Plants need photosynthesis to survive. The balanced chemical equation for photosynthesis is 6 CO2 + 6 H2O ? C6H12O6 + 6 O2. In English terms, this equation translates to six carbon dioxide plus six
-Measuring the pH of a solution (such as in the lab we had) could also help determine the rate of photosynthesis. You would need a much more specific pH meter, but generally, if pH goes down, the level of CO2 is higher, meaning more cellular respiration. Higher pH means there’s less CO2, so more photosynthesis.
The rate of photosynthesis is affected by environmental factors like light intensity, light wavelength, and temperature. This experiment will test the
The process of photosynthesis, by which light energy is used to convert inorganic compounds into organic substances with the release of oxygen, may be the most important biological event sustaining life (Keir et al. 2017). In the light-dependent reactions, the chloroplasts of a plant use the pigment chlorophyll to convert light energy into chemical energy. This energy is used to split water and produce oxygen (Eller et al. 2015). The energy is later used in the light independent reactions, where carbon dioxide (CO2) undergoes carbon fixation with the aid of enzyme rubisco, because it catalyses both carboxylation and oxygenation reactions and most of responses of photosynthesis to light, CO2, and temperature (John Evans 2013).
Abstract: The purpose of this lab is to separate and identify pigments and other molecules within plant cells by a process called chromatography. We will also be measuring the rate of photosynthesis in isolated chloroplasts. Beta carotene, the most abundant carotene in plants, is carried along near the solvent front because it is very soluble in the solvent being used and because it forms no hydrogen bonds with cellulose. Xanthophyll is found further from the solvent font because it is less soluble in the solvent and has been slowed down by hydrogen bonding to the cellulose. Chlorophylls contain oxygen and nitrogen and are bound more tightly to the paper than the other pigments.
Photosynthesis is a food making process for algae and plants. The photosynthesis process rate varies from different wavelengths and intensities of light. This lab will evaluate the optimal wavelengths and degrees of intensity during photosynthesis when chloroplast is exposed to light. The mixtures of DCPIP with water, PO4 buffer, and chloroplast will be prepared in a number of cuvettes. The cuvettes were tested individually at different wavelengths and intensities to find the optimal rate of photosynthesis by using a spectrophotometer, measuring the greatest change in absorbance. From this experiment, two data charts and four graphs were obtained. The hypothesis was set from graphs obtained in this lab, and the optimal reaction
photosynthesis happens in two stages: light reaction and carbon fixation also known as calvin cycle.light reaction TAKES PLACE IN THYLAKOID USE light energy to produces atp and nadph whereas, calvin cycle takes place instroma uses energy derived from light dependent reaction to make GA3P from CO2 ( Bio166 lab execise manual, 2015). the purpose of this experiment was to separate plant
For lab 12, it is hypothesized that chlorophylls a and b are present in a plant leaf and contribute to the starch production in photosynthesis. Also, products of photosynthesis will be present in leaf tissue exposed to red and blue light wavelengths for several days, but a decreased presence in leaf tissue exposed to green and black light wavelengths. In lab 13, it is expected that since chlorophylls a and b are more polar and smaller molecules than the anthyocyanins and carotenoids, they will travel higher up the chromatography paper than the other pigments.
Photosynthesis is a vital process that autotrophs use to transfer light energy into chemical energy. Photosynthesis ultimately produces O2 and glucose. It, like many other biological processes, can be affected by environmental variables. The variable that we altered in the following experiment are intensity, light wavelengths, and pigment types. In order to do this, we conducted three experiments. In the first experiment, we examined the effect of light intensity by placing vials with chloroplasts with DPIP at different light distances in which the results varied. Initially, 30cm away was the most effective for photosynthesis. Then 24cm appeared to be the most effective. Followed by 49cm at minutes 25 and 30. In the second experiment, we
The oxygen is released as a waste product, while the H+ and electrons are used to reduce NADP+ to NADPH. At the same time ADP is used to create ATP which is used as a source of energy for later parts of photosynthesis.
Photosynthesis has a two-stage performance before plants produce the two products they are known to produce. These stages are Photosystem I and II. Photosystem II is dependant on light reactions for energy which causes the electrons to be react and be transferred to Photosystem II. The electrons are transported through the Photosystem II electron transport system, however some energy is used to drive ATP synthesis. Meanwhile, light is being absorbed by the Photosystem I, which causes the electrons to react. This process sends the electrons to the Photosystem I transport system where some energy is released as electrons travel through the electron transport system and is captured as NADPH. When this process is completed oxygen is released from the plant and glucose has been
In photosynthesis, the dark reactions are chemical reactions that convert carbon dioxide and other compounds into glucose. These reactions unlike light-dependent reactions do not need light to occur. These reactions take the products of the light-dependent reactions and perform further chemical processes on