Photosynthesis

And finally into test tube 3, I pipetted 1.0 ml turnip extract and 4.0 ml of water. The contents of test tube 1 was poured into a spectrometer tube and labeled it “B” for blank. “B” tube was now inserted it into the spectrometer. An adjustment to the control knob was made to zero the absorbance reading on the spectrometer since one cannot continue the experiment if the spectrometer is not zeroed. A combination of two people and a stop watch was now needed to not only record the time of the reaction, but to mix the reagents in a precise and accurate manner. As my partner recorded the time, I quickly poured tube 3 into tube 2. I then poured tube 2 into the experiment spectrometer tube labeled “E” and inserted it into the spectrometer. A partner then recorded the absorbance reading for every 20 seconds for a total of 120 seconds. After the experiment, a brown color in the tube should be observed to indicate the reaction was carried out. Using sterile techniques, any excess liquid left was disposed

The purpose of this experiment is to determine the maximum absorbance of fast green, and the chlorophylls, also in the case of fast green create a concentration curve to determine an unknown substance. Each test will use the spectrophotometer.

Photosynthesis is the process in which plants consumed inorganic materials like solar light, carbon dioxide and water and converted it to an organic molecule like sugar and an inorganic gas like oxygen. Light is one of the major elements influencing the rate of photosynthesis; direct light concentration affects the noncyclic pathway (light

The ending result of this experiment confirms that as five test tubes are lined up with the varying level of absorbance, different results in the level of absorbance will appear as well, this is visible in above table. Thus, this is due to the varying amount of water in the solution. The blank sample had a 0.30 in its level of absorbance.

Photosynthesis is a process used by plants and other organisms to convert light energy into chemical energy that can later be released to fuel the organisms ' activities. Plants need photosynthesis to survive. The balanced chemical equation for photosynthesis is 6 CO2 + 6 H2O ? C6H12O6 + 6 O2. In English terms, this equation translates to six carbon dioxide plus six

14) Obtain the unboiled chloroplast suspension, mix, and transfer 3 drops to cuvette 3. Immediately cover and mix cuvette 3. Insert it into the spectrophotometer's sample holder, read the percentage transmittance, and record it in Table 4.4. Replace cuvette 3 into the incubation test tube rack. Take and record additional readings at 5, 10, and 15 minutes. Mix the cuvette's contents just prior to each readings. Remember to use cuvtte 1 occasionally to check and adjust the spectrophotometer to 100% transmittance.

In tube 3, the water was at 40. This is still warm but not the color was not nearly as intense as the previous tube. The next tube charted was tube number 4. The beet was subjected to a temperature of only 22°c. That temperature I would chart as “room temperature”. I found that the least amount of dye was leaked from the beet. For the cold methods I concluded that the amount of betacyanin that escaped from the cell membrane was intense, like the hot treatment results. I concluded that it didn’t have to be hot temperature stress to release betacyanin. Tube number 5 was placed into the refrigerator and the level of dye that permeated the water was charted at a 6. Tube 6 was placed in the freezer and was documented at a level of color intensity of a ten. Also, when the tube was pulled from the freezer the specimen has noticeably changed. It has a slight white, almost white frost or texture to it. Please see attachment and table below.

Using the yellow tube, which included everything but starch, as the blank, each group zeroed their spectrophotometer. This was done so that any absorbance observed depends only on the amount of starch present, not on any other reagents (buffer, IKI). To zero the spectrophotometer, the wavelength was first set at 580nm, using knob 3 (45). Next, the groups made sure that the light next to “transmittance” was lit, and the chamber to be tightly closed. Having the chamber empty & closed tightly provides reference for the darkest condition possible. Using knob 1, the transmittance was turned until it read 0.0 (45). Before the groups used their blank test tube to zero the spectrophotometer, each needed to wipe the tube with kimwipes to ensure a clean reading. Turning knob 2, each group was then instructed to zero the absorbance, 0.000. Upon removing the blank, each trial was inserted into the chamber (46). The

When the beetroot sample is heated for a longer period of time, it is exposed to the vigorous and frequent vibrating molecules for a longer duration. This further disrupts the cell membranes making them more fragile, thus more pigment will leak out into the external solution. With more leakage of the betacyanin pigment, the % transmittance should be lower, indicating that the % of light that is able to pass through the beetroot solution should be less as duration heated increases.

The initial experiment was a success. As our treatment group spent more and more time under the lights, the absorbance rate continues to decrease toward zero. Once our 30 minutes were up, the absorbance rate in each tube was significantly lower than at the start of our experiment. In contrast the two control groups did significantly lower the absorbance. Each control lacked one of the vital aspects of photosynthesis, one being light, and the other being chloroplast. Neither of the control groups (Control 1 or 2) showed any signs of photosynthesis. Control 1 was exposed to light, but contained no photosynthetic organelles thus the absorbance throughout the 30 minutes varied minimally, mostly staying stagnant. Control two which contained chloroplast but was not exposed to any light failed to lower the absorbance at all and in fact increased the absorbance over the 30 minutes. However, the treatment group contained both and ultimately performed photosynthesis as we expect therefore, confirming our assumption that chloroplast were the organelles required for photosynthesis in plants and that light is required to perform said photosynthesis. The treatment group, containing both the chloroplast and being exposed to light provided evidence that photosynthesis was taking place as the absorbance lowered at each 10-minute interval. Having a less absorbance would be desired because as DCIP became reduced we would expect the solution to become more and more clear, thus less

The absorbance is measured using a Plate reader and a Standard curve is generated. Also, the different types of pipetting techniques are assessed in this Assay.

We found that Tube 1 had an absorbance of 0.085, Tube 2 had 0.034, Tube 3 had 0.027, Tube 4 had 0.032, Tube 5 had 0.025, Tube 6 had 0.028, Tube 7 had 0.022, Tube 8 had 0.024, Tube 9 had 0.030, and Tube 10 had an absorbance of 0.022. We then graphed absorbance vs fraction number so that we could demonstrate the absorbance of each tube and found that Tube 1 had our highest value of absorbance. Basically, the first few tubes have the most protein and then the rest drop off as is shown in the graph.

Photosynthesis is essential to all living organism such as animals and plants. Photosynthesis is a process used by plants and other autotrophs to capture light energy and use it to power chemical reaction that converts carbon dioxide and water into oxygen, carbohydrates and water. (Textbook: Principles of Biology). The reactants and the products of photosynthesis are:

Photosynthesis is a huge concept to learn and understand in the field of biology. Plants have their own special way of using the ATP they produce. Photosynthesis is a process where plants harness the sunlight they receive and they produce carbohydrates, as well as oxygen for living things and other plants. Now the sunlight ultimately powers the process of

     Photosynthesis is a reproductive system that occurs in plants. The main components required for photosynthesizing are sunlight, energy, water and mineral from soil, and carbon dioxide from the air. Once these components are combined they