Tim is interested in analyzing for Pyraclostrobin (pesticide) in strawberries using QuECHers method for extraction and clean up before analysis with HPLC-uv. The concentration of the Pyraclostrobin in the fruits that he sampled is thought to be between 1-10 ng pesticide per g fruit fresh weight. The HPLC-uv instrument he is using has a LoQ of 5 ppb for Pyraclostrobin. Tim is starting out with 500 mg of the sliced fruit
Q: A chromatographic analysis is performed with a sample containing toluene and benzene. The column…
A:
Q: Which of the following would result in an increase in the calculated ngas in the Chem 16.1 Experin…
A: The correct option is as follows:
Q: 300.0 mg conc 0.100 M expID: HM1-001 scale 1) EDCI, HOBT hydrate DIPEA O`HN NH H3CO HN DCM:DMF (1:2)…
A: Since you have posted a question with multiple sub-parts, we will solve first three subparts for…
Q: in the separation of these 8 organic herbicides using HPLC in two different modes: Isocratic elution…
A: Solution: i) part Comparison of isocratic elution and gradient elution in this experiment: Isocratic…
Q: What is the retention time of peak 1 in this gas chromatogram? (mV] Clarity Lite - 8_11_2020 9_40_24…
A: The difference between the point of injection and the peak of the solute gives the retention time…
Q: Retention Time Area of Peak % of Total Identity Component 1 Component 2 Component 3
A: Given data:
Q: Which solution is of known concentration that is used to react completely with an analyte?…
A:
Q: As the temperature of a gas chromatography column increases, the the retention time of the analyte…
A: Gas chromatography is a technique used for the separation of compounds and analyzing the compound…
Q: Ibuprofen, an acidic drug, which has a pKa of 4.62, is analyzed by chromatography on C18 column with…
A: Ibuprofen is a medication in the nonsteroidal anti-inflammatory drug class that is used for treating…
Q: Which of the following best describes the application of flow analysis? O a Asample is injected into…
A: The flow injection method was developed by Ruzicka and Hansen in Denmark in the mid-1970. This is a…
Q: Jörgen et al. Extracted acrylamide from potato chips by soxhlet extraction. They used methanol as a…
A: From the unit (hours) given it can be concluded that time has been plotted in X-axis.
Q: The following data were obtained during gas-liquid chromatography of a mixture of two volatile…
A: Given data for components X and Y:
Q: A mixture of species of A, B, and C is injected into a liquid chromatographic column with length of…
A: Selectivity factors measure as the distance between two chromatography peak. Retention factor (k)…
Q: Gradient elution used in HPLC. How is the gradient created for a reverse-phase HPLC experiment over…
A: Given that Gradient elution is used in HPLC. To find: How is the gradient created for the…
Q: An analyst determines the Cl^- content of rain water with ion chromatography. The data for the…
A: There is a high probability that an analytical instrument will produce a signal noise whenever a…
Q: systematic
A: The separation of compounds under the influence of the mobile phase and the stationary phase is…
Q: A sample of drinking water was tested for Pb+ and was found to have a Pb²* concentration of 17.46…
A: Given the concentration of the unspiked sample, Cunspiked = 17.46 ppb Now 1.00 mL of 2.29 ppb Pb2+…
Q: (6)(Inorganic) In an ion exchange chromatography column, based on sulfonate groups, as shown in…
A: The anions are produced by the removal of hydrogen ion (H+) from a neutral compound such as…
Q: 1. What are the advantages and relevance of performing the following in a chemical pathology lab? i.…
A: A blank solution is a solution containing little to no analyte of interest, usually used to…
Q: Methodology: Make schematic diagram for the procedure below B. % SO3 determination Dry the soluble…
A: We need to make a schematic diagram for the given procedure.
Q: Internal standard. A solution containing 3.47 mM X (analyte) and 1.72 mM S (standard) gave peak…
A: (a) Calculate the response factor for X: Response factor=Area of analyte peak/Concentration of…
Q: You prepare the following MeOH standards from a 2000 ppm stock MeOH solution: Calibration Blank, 50…
A: We have to give the , formula & R2 value of given data and determine the linear working range…
Q: ▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min,…
A: Answer : Relative retention time = retention time of analyte / retention of known peak based on…
Q: The following figure indicated the separation 5-hydroxymethylfurfural (HMF), patulin (PAT),…
A: To predict the order of polarity of the given compounds.
Q: The detecton limits reported for Al with ICP-AES and GF-AAS are 2 ppb and 5 picograms, respectivly.…
A: Given:Al with ICP-AES = 2 ppb = 2 µg/L = 2×10-6 g/L.Mass of Al with GF-AAS = 5 picogram = 5×10-12…
Q: The following compounds were placed into a column of silica gel for liquid chromatography 1,3…
A: In silica chromatography, we use silica which is acidic in nature. All the basic compounds do not…
Q: An open tubular chromatography column is 28.7 m long with an inner diameter of 0.503 mm . The inside…
A: Note -Since you have posted a question with multiple sub-parts, we will solve first three sub-parts…
Q: A mixture of A and B is injected into a liquid chromatographic column with length of 45 cm. The…
A: given- length of the plate= L = 45 cm plate height = H = ?
Q: In one paper chromatography, the Rf for spots X and Y are 0.5 and 0.35 respectively. The solvent…
A: In paper chromatography Rf value is the ratio of distance travelled by compound to distance…
Q: Include the theory of how molecules interact with and elute from molecular stationary phase of GC GC…
A: The sample is first injected into a mobile phase present in gaseous state also called as the Carrier…
Q: An analyte has an Rf value of 0.25. What does that indicate about the solubility of the analyte in…
A:
Q: ▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min,…
A:
Q: When a Levy-Jennings graph for TSH was checked, the mean of results was found to have shifted by…
A: Ans. (a) This is a systematic type error (a constant shift in one direction). Systematic or bias…
Q: A sample of Melbourne drinking water was injected into an ion chromatograph. The anions were…
A: Given: Void time = 1.25 min ts for chlorides = 3.12 min ts for sulfate = 6.62 min
Q: An analyst was measuring the performance of a HPLC column. The test solute was Toluene (5 µL). The…
A: To find out the mobile phase velocity, number of theoretical plates and height equivalent to…
Q: 1. Sensitivity in a flow-injection analysis is directly proportional to the height of an analyte's…
A: Answer is option e Increase in flow rate, decreasing the length and diameter of tubing using a…
Q: Separation of Peaks Below are two chromatograms after LC separation. Which chromatogram is better to…
A: LCMS is Liquid chromatography–mass spectrometry which is used for mass analysis of unknown or known…
Q: what can be an analyte for using internal standard? sample: vegetable oil containing ethyl oleate,…
A: In the analytical techniques, such as gas chromatography or HPLC, standardization of the sample…
Q: IC An analyte has a Rf value of 0.50, What does that indicate about the solubility of the analyte in…
A: Retention factor describes the ratio of time spent in the stationary phase relative to time spent in…
Q: What is the role of stationary phase and mobile phase in Chromatography? Please shortly answer at…
A: The stationary phase remains fixed in place while the mobile phase carries the components of the…
Q: Which is NOT a factor that affects retention time? I. the pressure used II. The nature of the…
A: the retention time will vary depending on: the pressure used (because that affects the flow rate…
Q: Table 2. Effect of injection volume and temperature on retention time, peak width and resolution.…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Question 6 Which is NOT a factor that affects retention time? I. the pressure used II. The nature of…
A: The retention time will vary depending on: 1.The pressure used (because that affects the flow rate…
Q: Gradient elution used in HPLC. How is the gradient created for a reverse-phase HPLC experiment over…
A: High performance liquid chromatography or HPLC is an analytical technique used to identify and…
Q: A GC separation was conducted on a sample containing a 14) pesticide analyte, Pymetozine. This…
A: First we need to find out the RF value by using given infomation - AAN = 2791, CAN = 15.0ppm AIS =…
Q: Which statement regarding the techniques utilized in splitless injection for gas chromatographic…
A:
Q: Injection of ethane, propane, butane, pentane, and hexane on a squalene column under isothermal…
A:
Q: A sample of explosives residue is dissolved in an appropriate solvent and is injected into HPLC. The…
A: Introduction: Chromatography is a technique used to separate the mixture if solution. There are two…
Q: FT-IR technique can be utilized for the analysis of unknown analytes by matching it with__________.…
A:
Tim is interested in analyzing for Pyraclostrobin (pesticide) in strawberries using QuECHers method for extraction and clean up before analysis with HPLC-uv. The concentration of the Pyraclostrobin in the fruits that he sampled is thought to be between 1-10 ng pesticide per g fruit fresh weight. The HPLC-uv instrument he is using has a LoQ of 5 ppb for Pyraclostrobin. Tim is starting out with 500 mg of the sliced fruit
Step by step
Solved in 2 steps
- ▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min, and IS at 4 min. In the patient sample, the analyte elutes t 2 min and the IS at 8 min. What is the relative retention time and the analyte in the patient? A) methanol B) Ethanol chu C) IPAA young researcher set out to develop a method for quantifying the polycyclic aromatic hydrocarbon (PAH) content in a contaminated soil sample using open tubular gas chromatography. During the initial stages of method development, the researcher was unable to separate the early eluting compounds, anthracene and naphthalene, with baseline resolution. Select the possible modifications the researcher could make to improve the resolution. use a column with a smaller inner diameter decrease the column length increase the stationary phase thickness increase the temperature choose a different stationary phaseBelow is the procedure described for determination of paracetamol according to BP: "Weigh and powder 20 tablets. Add a quantity of powder equivalent to about 0.35 g paracetamol to 50 ml of 0.1 M NaOH, dilute with water to 200 ml and shake for 15 min. Add sufficient water to make up 250 ml. Filter the solution and dilute 5 ml of the filtrate to 100 ml with water. Dilute further 10 ml to 100 ml. Measure the absorbance at 257 nm. The A," following is correct? 715 at 257 nm". Which of the O a. An analyst can take 0.37g of the powdered tablets instead of 0.35. O b. An analyst can replace NaOH with HCI OC. An analyst can skip the shaking step O d. B andC O e. All of the above
- A sample of explosives residue is dissolved in an appropriate solvent and is injected into HPLC. The column contains a non- polar stationary phase. The sample revealed three chemical compounds that are commonly present in homemade explosives. ТAТP DADP HMTD 1 2 3 4 5 6 7 8 Retention time (minutes) a) determine the retention time of TATP. b) which one elute first :DADP,TATP or HMTD? c) State and explain which compound is most polar. d) If you are investigating this terrorist attacks, state one way in which the information in the chromatogram would be useful.Below is the procedure described for determination of paracetamol according to BP: "Weigh and powder 20 tablets. Add a quantity of powder equivalent to about 0.35 g paracetamol to 50 ml of 0.1 M NaOH, dilute with water to 200 ml and shake for 15 min. Add sufficient water to make up 250 ml. Filter the solution and dilute 5 ml of the filtrate to 100 ml with water. Dilute further 10 ml to 100 ml. Measure the absorbance at 257 nm. The A, = 715 at 257 nm". Which of the following is correct? O a. An analyst can take 0.37g of the powdered tablets instead of 0.35. O b. An analyst can replace NaOH with HCl O c. An analyst can skip the shaking step O d. B andC O e. All of the above▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min, and IS at 4 min. In the patient sample, the analyte elutes t 2 min and the IS at 8 min. What is the relative retention time and the analyte in the patient? ▪ A) methanol B) Ethanol ▪ C) IPA
- Below is the procedure described for determination of paracetamol according to BP: "Weigh and powder 20 tablets. Add a quantity of powder equivalent to about 0.35 g paracetamol to 50 ml of 0.1 M NaOH, dilute with water to 200 ml and shake for 15 min. Add sufficient water to make up 250 ml. Filter the solution and dilute 5 ml of the filtrate to 100 ml with water. Dilute further 10 ml to 100 ml. Measure the absorbance at 257 nm. The A = 715 at 257 nm". Which of the following is correct? O a. An analyst can take 0.37g of the powdered tablets instead of 0.35. O b. An analyst can replace NAOH with HCI C. An analyst can skip the shaking step d. B and C e. All of the aboveanalyte concentration(C)(mg/ml) injection volume (ul) elution time (time) peak DAD signal(mAU) caffeine 1 1 4.67 302.85 aspartame 5 1 7.53 15.83 benzoic acid 1 1 8.14 89.98 saccharin 1 1 1.91 84.86 mixture(add everything above with 1:1:1:1 ratio) 1 4.47 69.58 How to get the concentration of the mixture in this case?In a Bradford assay, 11 µl of a protein isolate sample was diluted by adding 21 µl of water prior to the addition 2.0 mL Bradford reagent. Calculate the protein concentration (in mg/mL) of the original protein isolate sample whose absorbance reading at 595 nm is 0.304 after dilution. The protein standard solutions used for the calibration curve of the Bradford assay was constructed using bovine serum albumin (BSA) stock solution whose concentration is 200 µg/mL. The concentration and the corresponding absorbance reading at 595 nm of the protein standard solutions are summarized in the table below: Standard soln # 1 2 13 4 6 conc of BSA 12.00 24.00 36.00 48.00 60.00 (pg/mL) Absorbance at 595 nm 0.000 0.156 0.249 0.328 0.411 0.528 zNote: Final answer format must be x.xxx (three decimal places). Round off only in the final answer. Do not round off in the middle of the calculation.
- During an assay of paracetamol by UV an analyst prepares a series of calibration solutions containing approximately 3-15 mcg/mL paracetamol as follows: 140.1 mg of pure paracetamol was added to a 200 mL volumetric flask. 0.1 M NaOH (50 mL) was added, the mixture well shaken and made up to volume with deionized water 10 mL of the above solution was diluted to 100 mL 1)What is the concentration of the final diluted solution in mcg/mL?(1 decimal) 2) What volume of the diluted solution is required to prepare 100 mL of a 8 mcg/mL standard solution? (1 decimal)25 ml of pickled brine was taken from an apple pickled with vinegar and diluted to 500 ml, 25 ml of this was taken and acidity was determined with 0.2 N (Factor: 1.05) NaOH. In the main trials, 6.3 ml - 6.5 ml - 6.9 ml, respectively, 0.1 ml base was used in the analysis without using the sample.For example, how many ppm is its acidity?The following data are from the HPLC assay of paracetamol 500 mg tablets using the calibration curve method: - Weight of active ingredient in powdered tablets taken for analysis: 180 mg • Initial extraction volume: 200 mL - Dilution steps: 10 mL into 100 mL, then 5 mL into 100 mL - Linear equation: y = 12000x - 50 %3! - Area of chromatographic peak for sample is 4750. What is the theoretical concentration in final solution in mg/100 mL? (2 decimal places). Please do not write the units, just numerical value. Answer: