2 9. Is it better to use a small injection volume or a large injection volume. In youranswer, explain what happens in the GC instrument that makes one better than theother? Table 2. Effect of injection volume and temperature on retention time, peakwidth and resolution.Peak Widthisopropanol(min)O5440.0531O.06150.06470.0649OvenRT MEOHPeak WidthRTResolutionMeOH (min) isopropanol(min)19041.7271.6961.7121.741Temp (°C) (min)60801001201401.5670.07200.03850.03190.0227-5921-6481.6837. Comment on the relationship between oven temperature and resolution for the 10uL injection. Is there any temperature at which the resolution is no longer sufficient togive baseline separation?8. Comment on and explain any differences that are observed in the chromatogram andtemperature dependence of the resolution between the 1 uL and 10 ul injectionvolumes.

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Table 2. Effect of injection volume and temperature on retention time, peak width and resolution. Peak Width isopropanol (min) O544 0.0531 1.06I5 0.0647 0.0649 Peak Width Resolution RT MeOH (min) isopropanol (min) 1904 1.727 1.696 1712 1.741 Oven RT MEOH Temp (°C) (min) 1.5670.0720 0.0385 0.0319 O.0227 60 80 1-592 100 1.648 120 1.683 140 7. Comment on the relationship between oven temperature and resolution for the 10 uL injection. Is there any temperature at which the resolution is no longer sufficient to give baseline separation? 8. Comment on and explain any differences that are observed in the chromatogram and temperature dependence of the resolution between the 1 ul and 10 ul injection volumes.

Table 2. Effect of injection volume and temperature on retention time, peak width and resolution. Peak Width isopropanol (min) O544 0.0531 1.06I5 0.0647 0.0649 Peak Width Resolution RT MeOH (min) isopropanol (min) 1904 1.727 1.696 1712 1.741 Oven RT MEOH Temp (°C) (min) 1.5670.0720 0.0385 0.0319 O.0227 60 80 1-592 100 1.648 120 1.683 140 7. Comment on the relationship between oven temperature and resolution for the 10 uL injection. Is there any temperature at which the resolution is no longer sufficient to give baseline separation? 8. Comment on and explain any differences that are observed in the chromatogram and temperature dependence of the resolution between the 1 ul and 10 ul injection volumes.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.1QAP

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Retention Time 40 20 25 Mntes VWD: Signal A, 284 nm Retention Area Identity Time (min) 7.867 3214697 Vitamin A 8.493 15687 19.410 140354 19.710 64346 20.803 884315 Vitamin E 21.640 36745 22.100 63054 What is the %composition of the Vitamin E peak in the sample? O 21.57% O 20.01% O 22.27% O 20.80% E8.403 89%花

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