Jörgen et al. Extracted acrylamide from potato chips by soxhlet extraction. They used methanol as a solyent for this and calculated the acrylamide yield in Gas Chromatography (GS-MS) by taking samples at certain day intervals. Accordingly, calculate the effect of the time parameter on the yield and the approximate % yield. 14000 12000 10000 8000 6000 4000 2000 50 100 150 200 250 Zaman (h) Acrylamide (ug/kg)
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- Table 1: Selected phytocomponents identified in the plant extract. Retention time, min Name of Compound 10.767 Benzaldehyde 15.790 Azulene 16.002 1-Piperazinecarboxaldehyde 17.935 Phthalan 20.731 2-methoxy-1,3,4-trimethybenzene 24.028 Methylesterpentanoic acid 26.993 Tetradecamethylcycloheptasiloxane 31.753 Hexadecamethyl Cyclooctasiloxane If GC is combined with MS, what data you will add as the 3rd column in Table 1 above. Fill in that data for each of the select identified phytocomponents.In order to improve the peptide separation by using a HPLC system, trifluoroacetic acid acts as mobile-phase modifier was added during the preparation of mobile phase. The preparation was performed by a postgraduate student as following:“2.851 g trifluoroacetic acid (MW: 114.02 g/mol) was made up to 500 cm3 in a graduated flask. To this solution, 50 cm3 of ethanol was added, and after mixing the mobile phase was placed in the solvent reservoir and pumping was commenced at 1.5 cm3 min-1.”Based on the given preparation procedure, identify THREE mistakes that were made.Use the following information collected from a SIZE-EXCLUSION CHROMATOGRAPHY experiment to answer the questions below. MW Standards Molecular Weight (kDa) Elution Volume (mL) blue dextran 1000 85 myoglobin 17.2 200 TIM 53.3 178 hemoglobin 62.2 156 IgG 140 122 ATCase 307.9 94 Prepare a graph showing the linear relationship between log function of molecular weight against Ve/Vo. The R2value must be 0.9 or higher. What is the linear equation from the standards given? What is the R2value?
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- Chemistry Briefly describe the differences between the scanning and SIM approaches with reference to your data. Discuss how and why your scanning (TIC) and SIM (mass chromatograms look different. Pay particular attention to selectivity of compounds detected and signal-to-noise. What are some advantages of SIM? What are some disadvantages?Explain why neutral solutes are eluted between times t0 and tmc in micellar electrokinetic capillary chromatography, where t0 is the elution time of neutral molecules in the absence of micelles and tmc is the elution time of the micelles.Help me.... Explain the principles of gel permeation chromatography in Polymer, what is the application in Polymer analytics
- Cite the applications for utilizing molecular/size exclusion chromatography in the analysis of samples. What are some of the limitations when using this analytical method?The following data give the recovery of bromide from spiked samples of vegetable matter, measuredusing a gas–liquid chromatographic method. The same amount of bromide was added to each specimen(Roughan, J.A., Roughan, P.A. and Wilkins, J.P.G., 1983, Analyst, 108: 742).Tomato: 777 790 759 790 770 758 764 ug/gCucumber: 782 773 778 765 789 797 782 ug/g(a) Test whether the recoveries from the two vegetables have variances which differ significantly.(b) Test whether the mean recovery rates differ significantly.i) To estimate the accuracy and precision of the method of determination of glucose in a blood sample, blood was spiked with a 88.2 ngml1 of glucose. The amount of glucose was determined in the spiked sample giving the following results in ngml1, 83.6, 69.0, 83.4 100.0 and 76.4. a)Find the accuracy of the quality control sample. b)Find the precision of the quality control sample