An analyst was measuring the performance of a HPLC column. The test solute was Toluene (5 µL). Thecolumn was a 4.6 mm i.d. column, 150 mm in length and packed with 5 µm C18 particles. The mobilephase was 40/60 water/methanol and the flow rate was 0.80 mL/min. The void time (to) was 1.86minutesThe retention time of the toluene sample was 14.50 min.The standard deviation of the peak was 7.0542 (s).2a. The mobile phase velocity was?0.0806 mm/s0.1344 mm/s1.344 mm/s1.344 x 10³ mm/s(i)(ii)(iv)2b. The number of theoretical plates was?(i)(ii)(iii)(iv)2c. The height equivalent to a theoretical plate was?1.041x10³ m1.015x105 m9.862x10-6 m1.186x10¹² m(i)(ii)(iii)(iv)4802152102470598482d. The reduced plate height was?(i)2.03(ii)1.97(iii)7.55(iv)2.082e. The column was?(i)(ii)Extremely well packedWell packedPoorly packed(iii)(iv) Insufficient information to decide about the quality of the column

To find out the mobile phase velocity, number of theoretical plates and height equivalent to…

An analyst was measuring the performance of a HPLC column. The test solute was Toluene (5 µL). The column was a 4.6 mm i.d. column, 150 mm in length and packed with 5 µm C18 particles. The mobile phase was 40/60 water/methanol and the flow rate was 0.80 mL/min. The void time (to) was 1.86 minutes The retention time of the toluene sample was 14.50 min. The standard deviation of the peak was 7.0542 (s). 2a. The mobile phase velocity was?

An analyst was measuring the performance of a HPLC column. The test solute was Toluene (5 µL). The column was a 4.6 mm i.d. column, 150 mm in length and packed with 5 µm C18 particles. The mobile phase was 40/60 water/methanol and the flow rate was 0.80 mL/min. The void time (to) was 1.86 minutes The retention time of the toluene sample was 14.50 min. The standard deviation of the peak was 7.0542 (s). 2a. The mobile phase velocity was?

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter26: An Introduction To Chromatographic Separations

Section: Chapter Questions

Problem 26.14QAP

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