A 5.00 mL sample of filtered orange juice was treated with 50.00 mL of 0.01019 M I3– to oxidize ascorbic acid to dehydroascorbic acid. After the oxidation was complete, excess I3– was back- titrated with 13.96 mL of 0.07125 M Na2S2O3 to starch indicator endpoint. What is the concentration of ascorbic acid (MM = 176.12) in the orange juice? Report the concentration ascorbic acid in mg/100mL.    C6H8O6 + I3– → C6H6O6 + 3I- + 2H+ I3– + 2S2O32- → S4O62- + 3I2

Introductory Chemistry: An Active Learning Approach
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ISBN:9781305079250
Author:Mark S. Cracolice, Ed Peters
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Chapter22: Biochemistry
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Problem 68E
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A 5.00 mL sample of filtered orange juice was treated with 50.00 mL of 0.01019 M I3 to oxidize ascorbic acid to dehydroascorbic acid. After the oxidation was complete, excess I3 was back- titrated with 13.96 mL of 0.07125 M Na2S2O3 to starch indicator endpoint. What is the concentration of ascorbic acid (MM = 176.12) in the orange juice? Report the concentration ascorbic acid in mg/100mL. 

 

C6H8O+ I3 → C6H6O+ 3I+ 2H+

I3 + 2S2O32- → S4O62- + 3I2

   

42.89 %

   

40.85 %

   

27.34 %

   

82.51 %

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