Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Textbook Question
Chapter 8.5, Problem 5CC
Retrieve, Infer, Apply
5. Which physical or chemical agent would be the best choice for sterilizing the following items: glass pipettes, tryptic soy broth tubes, nutrient agar, antibiotic solution, interior of a biological safety cabinet, wrapped package of plastic Petri plates? Explain your choices.
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Guide Questions:
Differentiate the types of culture media based on its physical state and uses.
What makes agar good support for microbial growth?
Cause-and-Effect Analysis: Given the conditions below, describe the effect on the staining procedure and explain briefly:
1. Excessive heat was applied during fixation
2. Low concentration of crystal violet used during gram staining
3. Excessive washing between steps
4. Insufficient decolorization
5. Excessive counterstaining
Activity 2. Media Used in Isolating Coliforms
You are a group of microbiologists tasked to test the presence of coliforms in the newly built water station. You are
asked to isolate E. coli, Salmonella, and Shigella. Enumerate the media used for each step of isolating these
bacteria. Include pictures and references.
Bacteria
Enrichment
(Broth/Agar)
Presumptive test
(Broth/Agar)
Isolation Media
(Broth/Agar)
E. coli
Salmonella
Shigella
1. How can you confirm that E. coli, Salmonella, and Shigella are present in the newly built water
station? Explain.
2. What is the difference between nutrient broth and nutrient agar?
3. What is the importance of these steps (Enrichment, Isolation, and presumptive test) in isolating bacteria?
Conclusion about the process of isolating bacteria
Chapter 8 Solutions
Prescott's Microbiology
Ch. 8.1 - MICRO INQUIRY Which types of agents can be used...Ch. 8.1 - Retrieve, Infer, Apply 1. Define the following...Ch. 8.1 - Retrieve, Infer, Apply 2. What is the difference...Ch. 8.2 - MICRO INQUIRY Examine graph (a). How long would it...Ch. 8.3 - MICRO INQUIRY How might one verify that filtration...Ch. 8.3 - Retrieve, Infer, Apply 1. What are depth filters...Ch. 8.3 - Retrieve, Infer, Apply 2. Describe the operation...Ch. 8.4 - Retrieve, Infer, Apply 1. Describe how an...Ch. 8.4 - Retrieve, Infer, Apply 2. In the past, spoiled...Ch. 8.4 - Prob. 3CC
Ch. 8.4 - Retrieve, Infer, Apply 2. What is the correlation...Ch. 8.5 - MICRO INQUIRY Why is it important that all of...Ch. 8.5 - MICRO INQUIRY Why are cross-linking agents such as...Ch. 8.5 - Retrieve, Infer, Apply 1. Why are most...Ch. 8.5 - Retrieve, Infer, Apply Construct a table that...Ch. 8.5 - Retrieve, Infer, Apply 3. Which disinfectants or...Ch. 8.5 - Retrieve, Infer, Apply 4. How do phenolic agents...Ch. 8.5 - Retrieve, Infer, Apply 5. Which physical or...Ch. 8.6 - Retrieve, Infer, Apply 1. Briefly explain how the...Ch. 8.6 - Retrieve, Infer, Apply How does being in a biofilm...Ch. 8.6 - Retrieve, Infer, Apply Suppose hospital custodians...Ch. 8.6 - Retrieve, Infer, Apply Briefly describe the phenol...Ch. 8.6 - Retrieve, Infer, Apply Why might it be necessary...Ch. 8.7 - Retrieve, Infer, Apply How would you explain to a...Ch. 8.7 - Retrieve, Infer, Apply Propose the use of specific...Ch. 8 - Prob. 1RCCh. 8 - Throughout history, spices have been used as...Ch. 8 - Design an experiment to determine whether an...Ch. 8 - Suppose you tested the effectiveness of...Ch. 8 - Prob. 4AL
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- Identify the method of sterilization/ chemical agent described This chemical agent is used as a standard for evaluating new chemical agents. This chemical agent is used as water disinfectant. A phenol-derivative that is less exotic and more potent than phenol. Considered the best antiseptic. Used as prophylaxis for ophthalmia neonatorum.arrow_forwardTopic: Pour-Plate Method 2. Molten agar mixed with bacterial suspension has a temperature higher than 45˚CEffect: Rationale:arrow_forwardBriefly compare filtration and ultraviolet (UV) radiation as antimicrobial methods.arrow_forward
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- Expert I just need sources with citations. Question:- Describe the mode of action beta-lactam, aminoglycosides, polyenes, and quinolone antimicrobial agents on bacterial cells. Provide 1 or 2 sources with the citations in APA formatarrow_forwardA Photo credit: BSP3D- Group 5 Pharm. Bio. Sci 3 Laboratory (S.Y. 2016-2017) Sample is Bacillus subtilis; A-Ciprofloxacin; B-Ampicillin; C-Tetracycline; D- Vancomycin Refer to the image above and answer the following questions: 1. By just looking at the example above, which do you think is the most effective antibiotic for Bacillus subtilis? which is the least effective? and why? 2. How will you measure the zone of inhibition of vancomycin (letter D)? 3. What is the importance of evenly distributing the microbe onto the plate? 3. Subtilisarrow_forwardTopic: Preparation of Microbial Suspension A Pure colony is needed for inoculation in sterile water, a small portion of culture media was included. Effect: Rationale:arrow_forward
- have a progressive su week. 1. Why are basic stains the most commonly used stains in microbiology? 2. What does the term "aseptic" mean? 3. Why are differential stains used more often than simple stains? 4. What are the three parts of a stain? 5. Why is it critical to allow cells to air dry before heat fixation? 6. Give an example of a basic stain and an acidic stain. 7. Give an example of a differential stain. 8. What is a medium? 9. What is the definition of a bacterial colony? 10. Why do we want to get an isolated bacterial colony from a mixed culture? Next lab: A continuation of staining techniques. to aarrow_forwardDISCUSSION Discuss sterilization technique Autoclave advantages? Autoclave tape? Filtrationarrow_forwardIN YOUR OWN WORDS. Differentiate the following. (Not more than 10 sentences) 1. Aseptic Teachnique vs. Sterile Technique 2. Microbistatic Agent vs. Microbicidal Agent 3. Sterilization vs. Disinfection 4.How is radiation, autoclave and ultrasonic waves used in the hospitals or clinics as a means to inhibit the growthbof microbes? 5. How is filtration applied nowadays in relation to Covid-19?arrow_forward
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