Title: Spoilage of coconut water Aim: To assess the diversity of psychrotrophic bacteria causing spoilage in coconut water. How might incubation of plates at 37°C influence the results of the quantitative dilution and plating of the coconut water samples?
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Title: Spoilage of coconut water
Aim: To assess the diversity of psychrotrophic bacteria causing spoilage in coconut water.
How might incubation of plates at 37°C influence the results of the quantitative dilution and plating of the coconut water samples?
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- Title: Spoilage of coconut water Aim: To assess the diversity of psychrotrophic bacteria causing spoilage in coconut water How might storage of the coconut water samples at room temperature after collection and before plating influence the results obtained from the samples incubated at 5ºC?Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?Loop with bacterial inoculum was subjected to flame sterilization prior to streaking on agar plate. Effect: Rationale:
- Can simply observing the colonies formed on agarplates and the results of a few biochemical testsconfirm how many different types of bacteria arepresent in coconut water samples? Explain.a.Describe what makes thioglycollate medium suitable for culturing anaerobes. What would the growth patterns of Clostridium sporogenes and Micrococcus luteus be in this medium? b. In the Kligler test, why do we inoculate the surface of the agar slope and then stab into the butt of the slope? What does a pink coloured colony indicate when using MAC (MacConkey Agar)?Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?
- A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/Lexplain how the use of an Eosin methylene blue agar plate can help determine the type of fermenters that bacteria are. explain throughly.The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?
- What is the major difference between an enrichment culture and a selective culture? Why are microbial doubling times in nature typically longer than those obtained in the lab? Briefly describe the following mechanisms of measuring bacterial growth: Direct microscopic cell count Plate count Most probable numberHow might incubating plates at 37°C influence the results of the quantitative dilution and plating of the coconut water samples?Is the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind of media based on what kind of microorganisms it allows to grow